Effect of epigallocatechin-3-gallate on radiation-induced esophagitis of rabbit

Q4 Medicine

中华放射医学与防护杂志 Pub Date : 2019-12-25 DOI:10.3760/CMA.J.ISSN.0254-5098.2019.12.004

Zhu Wanqi, Jia Li, Chen Guanxuan, Li Xiaolin, Xing Ligang, Zhao Han-xi, Y. Jinming

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Abstract

Objective To analyze the effect of epigallocatechin-3-gallate (EGCG) in radiation induced esophagitis of model rabbit. Methods Thirty male New Zealand rabbits were randomly divided into EGCG group, saline group, blank group. The rabbits in EGCG and saline groups were irradiated with 6 MV X-rays. The blank group did not receive radiation. After irradiation, rabbits were given with 440 μmol/L EGCG or saline three times a day in continuous 5 days. The scores of pathological changes of esophagus were observed by optical microscope.The serum levels of interleukine-1 beta (IL-1β), interleukine-6 (IL-6), transforming growth factor-β (TGF-β) and tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay. The expression levels of 67KD laminin receptor (67LR) was detected by immunohistochemistry. Results After treatment, the scores of pathological changes of esophagus in blank group, saline group, EGCG group were 0, 3.9±1.10 and 2.80±0.92, respectively. At different time points after drug treatment, the levels of serum inflammatory factors among three groups were significantly different (F=23.66-236.32, P<0.05). The expressions of 67LR in esophageal tissue of 3 groups were also significantly different (F=585.38, P<0.05). Conclusions EGCG reduced radiation-induced esophagitis in rabbits by decreasing the levels of serum inflammatory factors, which may be related to the expression of 67LR protein. Key words: Radiation injury; Epigallocatechin-3-gallate; Protection

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没食子儿茶素-3-没食子酸酯对兔放射性食管炎的影响

目的探讨没食子儿茶素-没食子酸酯(EGCG)对放射性食管炎模型兔的治疗作用。方法30只雄性新西兰兔随机分为EGCG组、生理盐水组、空白组。EGCG组和生理盐水组用6mv x射线照射。空白组不接受放射治疗。照射后给兔注射440 μmol/L EGCG或生理盐水，每天3次，连续5 d。光镜下观察食管病理变化评分。采用酶联免疫吸附法检测血清白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)、转化生长因子-β (TGF-β)、肿瘤坏死因子-α (TNF-α)水平。免疫组织化学检测67KD层粘连蛋白受体(67LR)的表达水平。结果治疗后，空白组、生理盐水组、EGCG组食管病理变化评分分别为0、3.9±1.10、2.80±0.92。药物治疗后不同时间点，三组患者血清炎症因子水平差异有统计学意义(F=23.66 ~ 236.32, P<0.05)。67LR在三组大鼠食管组织中的表达也有显著差异(F=585.38, P<0.05)。结论EGCG通过降低血清炎性因子水平，减轻兔放射性食管炎，其作用机制可能与67LR蛋白的表达有关。关键词:辐射损伤;Epigallocatechin-3-gallate;保护

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