Design Of Primer And Probe To Detect SNP Rs 1892901 In Fosl-1Gene In Different Types Of Cancer In Iraqi Population

Safa Ahmed Abed, Hiba Al-Khafaji
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引用次数: 5

Abstract

It has been shown that the etiology of thyroid, breast and prostate cancer is associated with hereditary and some environmental factors that cause damage to DNA. Protein coding genes are responsible for the development of protein and thus have been reported to be good candidate susceptibility genes for thyroid, breast and prostate cancer. (FOSL1) proteins have important functions in the growth, including the regulation of cell proliferation, differentiation and transformation. There have been studies of elevated levels of FOSL1 in cancers. However, the available methods for measuring FOSL1 levels are direct and quantitative using Poly Chain Reaction (RT-PCR) in real time. In the present study, whole blood was isolated from 100 individuals distributed into four groups as follows: Group 1 included: 25 samples from thyroid cancer patients; Group 2: 25 samples from breast cancer patients; Group 3: 25 samples from prostate cancer and Group 4:25 samples from apparently healthy individuals. The messenger RNA (mRNA) expression levels of FOSL_1in the peripheral blood was analyzed using reverse transcription polymerase chain reaction (RT PCR). The expression of FOSL_1 mRNA in the fold of gene expression in prostatic cancer group was 4 time higher than that of healthy group. That for the breast cancer group was 3 times higher than the healthy group and the thyroid cancer group was 3 times higher than the healthy group., respectively, Using GAPDH as Housekeeping Gene. In conclusion, there is an important link between blood and tumor tissue expression of FOSL1 gene. in thyroid, breast and prostate cancer, could allow the introduction in clinical practice of a simple test that would measure mRNA levels of DNA protein coding genes in peripheral blood samples instead of tissue samples; thus justifying its use as a prognostic and predictive factors in thyroid, breast and prostate cancer patients.
伊拉克人群不同类型肿瘤中化石-1基因SNP rs1892901的引物和探针设计
研究表明,甲状腺癌、乳腺癌和前列腺癌的病因与导致DNA损伤的遗传和一些环境因素有关。蛋白质编码基因负责蛋白质的发育,因此已被报道为甲状腺癌、乳腺癌和前列腺癌的良好候选易感基因。(FOSL1)蛋白在细胞生长过程中具有重要的功能,包括调控细胞增殖、分化和转化。已经有研究表明癌症中FOSL1水平升高。然而,现有的测量FOSL1水平的方法是实时直接定量的多聚链反应(RT-PCR)。在本研究中,从100个个体中分离全血,分为以下四组:第一组:甲状腺癌患者25个样本;第二组:25例乳腺癌患者标本;第3组:25个前列腺癌样本,第4组:25个健康个体样本。采用逆转录聚合酶链反应(RT PCR)分析外周血fosl_1信使RNA (mRNA)的表达水平。前列腺癌组基因表达折叠中FOSL_1 mRNA的表达量比健康组高4倍。乳腺癌组是健康组的3倍甲状腺癌组是健康组的3倍。分别使用GAPDH作为管家基因。综上所述,FOSL1基因在血液和肿瘤组织中的表达存在重要联系。在甲状腺癌、乳腺癌和前列腺癌中的应用,可以在临床实践中引入一种简单的测试,即测量外周血样本中DNA蛋白编码基因的mRNA水平,而不是组织样本;因此,证明其作为甲状腺癌、乳腺癌和前列腺癌患者的预后和预测因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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