The hepato-fibrogenic potential of both acute and chronic treatments with paracetamol, ibuprofen, and aspirin in rats

Journal of Bioscience and Applied Research Pub Date : 2022-10-01 DOI:10.21608/jbaar.2022.261227

N. Abdel-Hamid, M. Abdel Hamid, A. Mohamed

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Abstract

Background and purpose: Hepatotoxicity from frequently prescribed drugs has become an evolving health problem. This study was conducted to evaluate the risk of acute and chronic administration of acetaminophen (AAP), ibuprofen (Ibu), and acetylsalicylic acid (ASA). Methods: One hundred and twenty male albino rats, were divided into 2 main groups for acute and chronic study. Each group was sub-classified into 5 sub-groups (12 rats for each). Acute study: control (normal saline), AAP (single oral dose, 540 mg/kg, bw), AAP +Zn (APP and Zn ,227 mg/liter drinking water 24 hours before AAP administration), Ibu (single oral dose,440 mg/kg, bw), and ASA (single intraperitoneal dose,540 mg/kg, bw). Chronic (period for 60 days): control (normal saline), AAP (single daily doses, 48 mg/kg, bw), AAP +Zn (APP and Zn, 227 mg/liter drinking water for 6o days), Ibu (single daily doses, 48 mg/kg, bw), and ASA (single daily intraperitoneal doses, 40 mg/kg,). Results : Hepatic aminotransferases, alkaline phosphatase, isocitrate dehydrogenase, serum glycosaminoglycans, tissue hydroxyproline, and malondialdehyde were significantly elevated, but glutathione was significantly decreased, in both acute and chronic treatments in all treated groups. Prior treatment with Zn couldn’t change the effects of AAP, except on oxidative stress. Tissue changes after chronic treatment varied from fatty changes to vascular congestions and inflammation. Conclusion : We assume that both acute and chronic administration of AAP, Ibu, and ASA have deleterious hepatotoxic and fibrogenic effects on the liver with a non-significant protective role to Zn co-administration with AAP against oxidative stress. analysis of variance; acetylsalicylic acid; AST: aspartate aminotransferase; GAGAs: glucose amino glycans; reduced glutathione; HαE: α eosin; hydroxyproline; ibuprofen; ICDH: isocitrate dehydrogenase; malondialdehyde; NPCs: non-parenchymal OCT:

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大鼠急性和慢性对乙酰氨基酚、布洛芬和阿司匹林治疗的肝纤维化潜力

背景与目的:常用处方药引起的肝毒性已成为一个不断发展的健康问题。本研究旨在评估急性和慢性给药对乙酰氨基酚(AAP)、布洛芬(Ibu)和乙酰水杨酸(ASA)的风险。方法:雄性白化病大鼠120只，分为急性组和慢性组。每组再分为5个亚组，每组12只大鼠。急性研究:对照组(生理盐水)、AAP(单次口服，540 mg/kg, bw)、AAP +Zn (APP和Zn, AAP给药前24小时饮用水227 mg/l)、Ibu(单次口服，440 mg/kg, bw)和ASA(单次腹腔注射，540 mg/kg, bw)。慢性(持续60天):对照组(生理盐水)、AAP(每日单次剂量，48 mg/kg，体重)、AAP +Zn (APP和Zn, 227 mg/l饮用水，持续60天)、Ibu(每日单次剂量，48 mg/kg，体重)和ASA(每日单次腹腔注射剂量，40 mg/kg，体重)。结果:肝转氨酶、碱性磷酸酶、异柠檬酸脱氢酶、血清糖胺聚糖、组织羟脯氨酸、丙二醛在急慢性治疗组均显著升高，而谷胱甘肽显著降低。除对氧化应激的影响外，先前的Zn处理不能改变AAP的作用。慢性治疗后的组织变化从脂肪变化到血管充血和炎症不等。结论:我们认为急性和慢性给药AAP、Ibu和ASA对肝脏都有有害的肝毒性和纤维化作用，而锌与AAP共给药对氧化应激的保护作用不显著。方差分析;乙酰水杨酸;AST:天冬氨酸转氨酶;GAGAs:葡萄糖氨基聚糖;减少谷胱甘肽;HαE: α伊红;羟脯氨酸;布洛芬;ICDH:异柠檬酸脱氢酶;丙二醛;非实质OCT:

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Journal of Bioscience and Applied Research

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