Determination of Inhibitory Effects of Allium sativum Extract on Biofilm Production by Clinical Staphylococcus aureus Isolates

Tribhuvan University Journal of Microbiology Pub Date : 2021-12-31 DOI:10.3126/tujm.v8i1.41200

Ashim Rai, Meha Raj Banjara

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Abstract

Objectives: To determine anti-biofilm effect of fresh garlic extract (FGE) on Staphylococcus aureus biofilm production and the relationship between methicillin resistance and biofilm production intensity. Methods: Clinical S. aureus isolates were identified methicillin resistant S. aureus (MRSA) by cefoxitin disc diffusion method. The anti-biofilm effects of FGE on S. aureus biofilm biomass determination was done using crystal violet assay. Results: Among 71 S. aureus isolates, MRSA were 37 (52.1%). Among biofilm producers, comparison of biofilm biomass (absorbance at 492 nm) showed no significant differences in biofilm formation ability between MRSA and MSSA (p=0.325). Use of 10% FGE decreased biofilm production in MRSA and MSSA by 40.4% (p<0.001) and 48.1% (p<0.001) respectively. Detachment assay using sodium dodecyl sulfate showed that control group biofilm biomass was decreased by 41.2%, while test group was decreased by 61.7% (p<0.001). Conclusion: Garlic extracts has potency as an anti-biofilm agent and could be developed and used to manage different S. aureus biofilm related infections.

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大蒜提取物对临床分离金黄色葡萄球菌生物膜抑制作用的测定

目的:探讨鲜大蒜提取物(FGE)对金黄色葡萄球菌生物膜生成的抗生物膜作用，以及甲氧西林耐药性与生物膜生成强度的关系。方法:采用头孢西丁圆盘扩散法对临床分离的耐甲氧西林金黄色葡萄球菌(MRSA)进行鉴定。采用结晶紫法测定了FGE对金黄色葡萄球菌生物膜生物量的抑制作用。结果:71株金黄色葡萄球菌中，MRSA 37株(52.1%);在产生生物膜的菌株中，比较生物膜生物量(492nm吸光度)发现MRSA和MSSA的生物膜形成能力无显著差异(p=0.325)。10% FGE的使用使MRSA和MSSA的生物膜产量分别下降40.4% (p<0.001)和48.1% (p<0.001)。十二烷基硫酸钠分离实验显示，对照组生物膜生物量下降41.2%，试验组生物膜生物量下降61.7% (p<0.001)。结论:大蒜提取物具有抗生物膜作用，可开发用于各种金黄色葡萄球菌生物膜相关感染的治疗。

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Tribhuvan University Journal of Microbiology

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