Improved in vitro endothelialization on nanostructured titania with tannin/glycosaminoglycan-based polyelectrolyte multilayers.

IF 2.4
In vitro models Pub Date : 2022-06-03 eCollection Date: 2022-06-01 DOI:10.1007/s44164-022-00024-x
Roberta M Sabino, Matt J Kipper, Alessandro F Martins, Ketul C Popat
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Abstract

Purpose: Blood compatibility of cardiovascular implants is still a major concern. Rapid endothelialization of these implant surfaces has emerged as a promising strategy to enhance hemocompatibility and prevent complications such as thrombus formation and restenosis. The successful endothelialization of implant surfaces mostly depends on the migration of endothelial cells (ECs), the differentiation of stem cells, and the inhibition of smooth muscle cell (SMC) proliferation. Our previous study demonstrated that nanostructured titania surfaces modified with polyelectrolyte multilayers based on tanfloc (a cationic tannin derivative) and glycosaminoglycans (heparin and hyaluronic acid) have improved antithrombogenic properties.

Methods: In this work, we used in vitro cell culture of ECs and SMCs to investigate the outcomes of these surface modifications on endothelialization. The cells were seeded on the surfaces, and their viability, adhesion, and proliferation were evaluated after 1, 3, and 5 days. Indirect immunofluorescent staining was used to determine the cellular expression of ECs through the presence of specific marker proteins after 7 and 10 days, and EC migration on the NT surfaces was also investigated.

Results: The surfaces modified with tanfloc and heparin showed enhanced EC adhesion, proliferation, and migration. However, SMC proliferation is not promoted by the surfaces. Therefore, these surfaces may promote endothelialization without stimulating SMC proliferation, which could improve the hemocompatibility without enhancing the risk of SMC proliferation leading to restenosis.

Conclusions: The surface modification here proposed is a promising candidate to be used in cardiovascular applications due to enhanced antithrombogenic and endothelialization properties.

单宁/糖胺聚糖基聚电解质多层膜改善纳米结构二氧化钛的体外内皮化。
目的:心血管植入物的血液相容性仍然是一个主要的问题。这些植入物表面的快速内皮化已成为增强血液相容性和预防血栓形成和再狭窄等并发症的一种有前途的策略。移植体表面的成功内皮化主要取决于内皮细胞(ECs)的迁移、干细胞的分化和平滑肌细胞(SMC)增殖的抑制。我们之前的研究表明,纳米结构的二氧化钛表面被基于tanfloc(一种阳离子单宁衍生物)和糖胺聚糖(肝素和透明质酸)的聚电解质多层修饰,具有改善的抗血栓形成性能。方法:采用体外培养的方法对内皮细胞和间充质干细胞进行表面修饰,观察其对内皮化的影响。分别于1、3、5天后观察细胞的生存力、粘附力和增殖能力。在7天和10天后,通过特异性标记蛋白的存在,采用间接免疫荧光染色法检测EC的细胞表达,并研究EC在NT表面的迁移。结果:经tanfloc和肝素修饰的细胞表面具有增强EC粘附、增殖和迁移的作用。然而,表面并不能促进SMC的增殖。因此,这些表面可能促进内皮化而不刺激SMC增殖,从而改善血液相容性而不增加SMC增殖导致再狭窄的风险。结论:本文提出的表面修饰由于具有增强的抗血栓形成和内皮化特性,在心血管应用中具有很好的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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