Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts

Abderrahim Benslama, Abdenassar Harrar
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引用次数: 38

Abstract

The aim of this study is to evaluate antioxidant activity of aqueous (Aq.E) and methanolic (Met.E) extracts of Zygophyllum album L. (Zygophyllaceae) and Arthrophytum scoparium (Chenpodiacea), two medicinal plant from Algerian Sahara used in traditional pharmacopoeia. The results showed that Aq.E have the heights yields 24% and 22.3% for Z. album and A. scoparium respectively. Qualitative analysis of showed that the Met.E of Z. album of had the highest amount of polyphenols (202.26±4.73 μg GAE/mg E) and flavonoids (15.77±0.94 μg QE/mg E). The antioxidant activity of extracts was evaluated by 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay. Results showed that the extracts of A. scoparium. Have the highest free radical scavenger activity in DPPH test (EC50=0.096±0.001 and 0.108±0.002 mg/ml for Met.E and Aq.E respectively). In addition, the results of reducing power assay demonstrated that Met.E of Z. album had the highest effect with 2399.65±12.31 μg ascorbic acid equivalent/mg E.Our results showed that the methanolic extract reported a considerable free radicals scavenging activity and reducing effect, may be due to their richness on polyphenols and flavonoids.
两种阿尔及利亚撒哈拉药用植物提取物的自由基清除活性和还原力
研究了阿尔及利亚撒哈拉地区传统药典中常用的两种药用植物刺槐科(zgophyllum album L.)和刺槐节植物(Arthrophytum scoparium, Chenpodiacea)的水提物(Aq.E)和甲醇提物(Met.E)的抗氧化活性。结果表明,Aq.E对黄芪和黄芪的高收率分别为24%和22.3%。定性分析表明,大都会。其中,黄酮类化合物含量最高(15.77±0.94 μ QE/mg E),多酚含量最高(202.26±4.73 μ QE/mg E), 2,2′-二苯基-1-苦味酰肼(DPPH)和还原力测定法评价了提取物的抗氧化活性。结果表明,黄芩提取物;在DPPH试验中,Met的EC50分别为0.096±0.001和0.108±0.002 mg/ml,对自由基的清除活性最高。E和Aq.E分别)。此外,还原功率试验结果表明,Met。结果表明,黄芪提取物的抗坏血酸含量为2399.65±12.31 μg /mg,具有较强的抗坏血酸清除活性和降低自由基的作用,这可能与黄酮类和多酚类物质丰富有关。
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