Mechanism of thiosulfate oxidation by Thiobacillus novellus

A. Michael Charles , Isamu Suzuki
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引用次数: 94

Abstract

  • 1.

    1. Whole cells and cell-free extracts of Thiobacillus novellus oxidized thiosulfate to sulfate without intermediary accumulation of sulfur of polythionates, consuming 2 moles of O2 for every mole of thiosulfate oxidized.

  • 2.

    2. Tetrathionate was oxidized to sulfate by whole cells, but not by cell-free extracts.

  • 3.

    3. Sulfite was rapidly oxidized by whole cells and extracts to sulfate, consuming 0.5 mole of O2 for every mole of sulfite oxidized. The oxidation was catalyzed by sulfite oxidase (sulfite: cytochrome c oxidoreductase) and cytochrome oxidase (cytochrome c: O2 oxidoreductase, EC 1.9.3.1) as shown by manometric as well as spectrophotometric experiments.

  • 4.

    4. Elemental sulfur was oxidized by whole cells to sulfate, but the oxidation by extracts required the addition of GSH.

  • 5.

    5. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was found in the extracts which reduced cytochrome c within thiosulfate only in the presence of cyanide.

  • 6.

    6. The following reactions are proposed for the oxidation of thiosulfate by T. novellus:

新硫杆菌氧化硫代硫酸盐的机理
1.1. 新硫杆菌(Thiobacillus novellus)的全细胞和无细胞提取物将硫代硫酸盐氧化为硫酸盐,没有中间聚硫酸盐的硫积累,每氧化1mol硫代硫酸盐消耗2mol O2。四硫酸盐被全细胞氧化为硫酸盐,而非细胞提取物则不能。亚硫酸盐被整个细胞和提取物迅速氧化为硫酸盐,每氧化1mol亚硫酸盐消耗0.5 mol O2。实验结果表明,亚硫酸盐氧化酶(亚硫酸盐:细胞色素c氧化还原酶)和细胞色素氧化酶(细胞色素c: O2氧化还原酶,EC 1.9.3.1)催化了氧化反应。单质硫被全细胞氧化为硫酸盐,但被提取物氧化需要添加GSH.5.5。罗丹斯(硫代硫酸盐:氰化物硫转移酶,EC 2.8.1.1)仅在氰化物存在的情况下才能在硫代硫酸盐中还原细胞色素c。本文提出了以下几种反应来催化T. novellus氧化硫代硫酸盐:
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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