{"title":"Mechanism of thiosulfate oxidation by Thiobacillus novellus","authors":"A. Michael Charles , Isamu Suzuki","doi":"10.1016/0926-6593(66)90012-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Whole cells and cell-free extracts of <em>Thiobacillus novellus</em> oxidized thiosulfate to sulfate without intermediary accumulation of sulfur of polythionates, consuming 2 moles of O<sub>2</sub> for every mole of thiosulfate oxidized.</p></span></li><li><span>2.</span><span><p>2. Tetrathionate was oxidized to sulfate by whole cells, but not by cell-free extracts.</p></span></li><li><span>3.</span><span><p>3. Sulfite was rapidly oxidized by whole cells and extracts to sulfate, consuming 0.5 mole of O<sub>2</sub> for every mole of sulfite oxidized. The oxidation was catalyzed by sulfite oxidase (sulfite: cytochrome <span><math><mtext>c</mtext></math></span> oxidoreductase) and cytochrome oxidase (cytochrome <span><math><mtext>c</mtext></math></span>: O<sub>2</sub> oxidoreductase, EC 1.9.3.1) as shown by manometric as well as spectrophotometric experiments.</p></span></li><li><span>4.</span><span><p>4. Elemental sulfur was oxidized by whole cells to sulfate, but the oxidation by extracts required the addition of GSH.</p></span></li><li><span>5.</span><span><p>5. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was found in the extracts which reduced cytochrome <span><math><mtext>c</mtext></math></span> within thiosulfate only in the presence of cyanide.</p></span></li><li><span>6.</span><span><p>6. The following reactions are proposed for the oxidation of thiosulfate by <em>T. novellus</em>: <figure><img></figure><figure><img></figure><figure><img></figure><figure><img></figure></p></span></li></ul><figure><img></figure></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":"128 3","pages":"Pages 510-521"},"PeriodicalIF":0.0000,"publicationDate":"1966-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90012-9","citationCount":"94","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926659366900129","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 94
Abstract
1.
1. Whole cells and cell-free extracts of Thiobacillus novellus oxidized thiosulfate to sulfate without intermediary accumulation of sulfur of polythionates, consuming 2 moles of O2 for every mole of thiosulfate oxidized.
2.
2. Tetrathionate was oxidized to sulfate by whole cells, but not by cell-free extracts.
3.
3. Sulfite was rapidly oxidized by whole cells and extracts to sulfate, consuming 0.5 mole of O2 for every mole of sulfite oxidized. The oxidation was catalyzed by sulfite oxidase (sulfite: cytochrome oxidoreductase) and cytochrome oxidase (cytochrome : O2 oxidoreductase, EC 1.9.3.1) as shown by manometric as well as spectrophotometric experiments.
4.
4. Elemental sulfur was oxidized by whole cells to sulfate, but the oxidation by extracts required the addition of GSH.
5.
5. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was found in the extracts which reduced cytochrome within thiosulfate only in the presence of cyanide.
6.
6. The following reactions are proposed for the oxidation of thiosulfate by T. novellus:
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