Study on screening Vietnamese herbs with antiviral activity to create products to support treatment of some diseases caused by RNA-virus

Abstract

Using information technology and traditional oriental medicine research, we have screened 5 Vietnamese herbs that contain active ingredients with antiviral effects. Andrographispaniculata, Syzygiumaromaum, Zingiber officinale Rose, Houttuyniacordata, Glycyrrhizauralensis Fisch. The active ingredients in herbs are extracted by ultrasound in a water-ethanol solvent system and made in the form of a nanometer complex, then mixed in specified proportions to form the product. The product was evaluated cytotoxicity by MTT (3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay and evaluated the growth inhibition effect of H5N1 virus. Result: The complex of active ingredients in the composition has the nanometer size, the main size is 443 nm, the zeta potential is -11.9 mV. The inhibitory activity of the H5N1 virus was dose-dependent, and a concentration of 3 mg/mL completely inhibited the growth of the H5N1 virus in the erythrocyte agglutination test.