Antibacterial Activity of Biologically Synthesized Nanosilver against Drug-Resistant Bacterial Pathogens

M. Dubey, S. Bhadauria, V. Sharma, V. Katoch
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引用次数: 1

Abstract

ABSTRACT Biosynthesis of nanoparticles by plant extracts is currently under exploration. Biological methods are a good competent to chemical procedures, which are environmentally friendly and convenient. We report the use of new plant extract of Cuscuta reflexa in the extracellular biosynthesis of silver nanoparticles. Bioactive silver nanoparticle synthesis was achieved by reacting the biomass of C. reflexa with aqueous solutions of silver nitrate (AgNO3) at ambient temperature. The formation of silver nanoparticles was confirmed by ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction patterns, and transmission electron microscopy (TEM). In the present study we report the excellent antibacterial activity of silver nanoparticle (with C. reflexa extract) against gram-positive and gram-negative multi-drug-resistant (MDR) bacterial strains using a zone of inhibition method. Scanning electron microscopy (SEM) imaging studies revealed that silver nanoparticles physically damaged the bacterial cell and ...
生物合成纳米银对耐药病原菌的抑菌活性研究
利用植物提取物生物合成纳米颗粒是目前研究的热点。生物法具有环境友好、操作方便等优点,可以很好地代替化学法。我们报道了一种新的植物提取物在银纳米粒子的细胞外生物合成中的应用。以C. reflexa生物量与硝酸银(AgNO3)水溶液在常温下反应,合成了具有生物活性的纳米银颗粒。通过紫外可见光谱(UV-Vis)、x射线衍射图和透射电子显微镜(TEM)证实了银纳米颗粒的形成。在本研究中,我们报道了银纳米颗粒(含C. reflexa提取物)对革兰氏阳性和革兰氏阴性多重耐药(MDR)菌株的良好抑菌活性。扫描电镜(SEM)成像研究表明,银纳米颗粒对细菌细胞有物理破坏作用。
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