Super Liquid-repellent Surfaces and 3D Spheroids Growth.

IF 3.1 4区生物学 Q2 Immunology and Microbiology

Frontiers in Bioscience-Landmark Pub Date : 2022-05-05 DOI:10.31083/j.fbl2705144

M. Ferrari, Francesca Cirisano, M. Morán

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引用次数: 3

Abstract

Substrates composition and surface features of materials rule adhesion control of cells to surfaces. As a result, most of the aspects of cell functions, such as spreading, migration, proliferation, and differentiation, can be significantly influenced in biomedical applications. Cell cultures make possible to understand cell biology, tissue morphology, mechanisms of diseases, drug action, and tissue engineering development, among others. Recent techniques related to culturing 3D cell aggregates in the presence of very low wettable surfaces represent an innovative field for in vitro experimentation aimed at more reliable conditions to investigate both tumor and non-tumor cell lines. Matching in particular cell biology to innovative materials, this work reviews the recent literature available on promoting cell aggregates formation strongly influenced by the high surface hydrophobicity. In particular, for spheroid formation, the highest water repellent coatings seem to be required for the significant effectiveness of the process. In this way, 3D cell culture has become a reliable method for reproducing in vitro cellular growth in more realistic physiological conditions.

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超级拒液表面和3D球体生长。

基质的组成和材料的表面特性决定了细胞对表面的粘附控制。因此，在生物医学应用中，细胞功能的大多数方面，如扩散、迁移、增殖和分化，都可能受到显著影响。细胞培养使了解细胞生物学、组织形态、疾病机制、药物作用和组织工程发展等成为可能。最近，在非常低的可湿性表面上培养3D细胞聚集体的技术代表了体外实验的一个创新领域，旨在更可靠的条件下研究肿瘤和非肿瘤细胞系。在特定的细胞生物学与创新材料相匹配的情况下，本工作回顾了最近关于高表面疏水性强烈影响下促进细胞聚集体形成的文献。特别地，对于球形的形成，最高的防水涂层似乎需要显著的有效性的过程。通过这种方式，3D细胞培养已经成为在更真实的生理条件下复制体外细胞生长的可靠方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Bioscience-Landmark 生物-生化与分子生物学

CiteScore

3.40

自引率

3.20%

发文量

301

审稿时长

3 months

期刊介绍： FBL is an international peer-reviewed open access journal of biological and medical science. FBL publishes state of the art advances in any discipline in the area of biology and medicine, including biochemistry and molecular biology, parasitology, virology, immunology, epidemiology, microbiology, entomology, botany, agronomy, as well as basic medicine, preventive medicine, bioinformatics and other related topics.