Effect of Activated Carbon on Rooting of Tissue Culture Seedlings of Qiuzi Pear

Xiao-feng Luan, Hao-wen Shi, B. Xu, Qiannan Zhang, Li Liu
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Abstract

In order to improve the rooting rate of Qiuzi pear tissue culture seedlings, this study took ‘sorb’ as experimental material, based on Qiuzi pear rooting tissue culture medium (1/2MS+IBA 0.5 mg/L), and added different concentrations of activated carbon (AC): 0.25 g/L, 0.50 g/L, 0.75 g/L, 1.00 g/L. The results showed that the rooting rate, rooting number and root length of tissue culture seedlings cultured with activated carbon were significantly higher than those of the control group. The most suitable medium for rooting Qiuzi pear was 1/2MS+IBA 0.5 mg/L+AC 1.00 g/L, and the rooting rate of the treated ‘sorb’ tissue culture seedlings reached 90%, which could obviously promote the growth of Qiuzi pear tissue culture. The results provide a theoretical basis for improving the rooting rate and survival rate of tissue culture seedlings of pear plants, and provide a reference for the establishment of rapid propagation system of woody plants.
活性炭对秋子梨组培苗生根的影响
为了提高秋子梨组培苗的生根率,本研究以‘sorb’为实验材料,在秋子梨组培苗生根培养基(1/2MS+IBA 0.5 mg/L)的基础上,添加不同浓度的活性炭(AC): 0.25 g/L、0.50 g/L、0.75 g/L、1.00 g/L。结果表明,活性炭培养组培苗的生根率、生根数和根长均显著高于对照组。最适合秋子梨生根的培养基为1/2MS+IBA 0.5 mg/L+AC 1.00 g/L,处理过的“sorb”组培苗生根率达到90%,能明显促进秋子梨组培苗的生长。研究结果为提高梨植株组培苗的生根率和成活率提供了理论依据,并为木本植物快速繁殖体系的建立提供了参考。
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