Metabolite Profiles and Antioxidant Activity of Caulerpa racemosa with Different Handlings

Q3 Environmental Science

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2018-12-30 DOI:10.15578/SQUALEN.V13I3.355

S. Sihono, K. Tarman, H. Madduppa, H. Januar

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引用次数: 9

Abstract

Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K10, K11, K12, and K13), while seawater handling extract contained very polar (K1, K2 and K3), polar (K6, K7, and K8) and low polar (K13) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC50 below 2,000 ppm) in ethanolic extract of C. racemosa. Keywords: antioxidant activity, Caulerpa racemosa, ethanolic extract,handlings, IC50

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不同处理总状茎的代谢谱及抗氧化活性

研究了不同处理总状茎叶提取物的代谢谱和抗氧化活性。在运输过程中，样品采用了三种不同的处理方式，即冰冷藏、液氮储存和海水浸泡。不同处理方式对乙醇粗提物和无机馏分收率影响显著，对有机馏分收率影响不显著。不同处理对总状花提取物的主要组分有不同的影响。低温处理样品的主要组分含有低极性组分(K10、K11、K12和K13)，而海水处理提取液含有极极性组分(K1、K2和K3)、极极性组分(K6、K7和K8)和低极性组分(K13)。在液氮中处理的样品萃取物含有平衡的馏分。总状叶乙醇提取物的抗氧化活性最高(IC50值低于2000 ppm)。关键词:抗氧化活性，总状茎，乙醇提取物，处理，IC50

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