Ladapa Kumdhitiahutsawakul, T. Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Y. Tragoolpua, S. Bovonsombut
{"title":"Biosurfactant-Producing Bacteria and Microbial Analysis in Mae Soon Reservoir, Fang Oil Field, Chiang Mai, Thailand","authors":"Ladapa Kumdhitiahutsawakul, T. Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Y. Tragoolpua, S. Bovonsombut","doi":"10.12982/cmjs.2023.024","DOIUrl":null,"url":null,"abstract":"Microbial Enhanced Oil Recovery (MEOR) is an effective alternative method for oil recovery in reservoirs using microorganisms or their secondary metabolites. This research aimed to evaluate the indigenous bacteria from Mae Soon reservoir by culture-dependent and culture-independent methods and to investigate the potential of biosurfactant-producing bacteria using a drop-collapsed assay. Indigenous bacteria were isolated from the oil sands of the reserved core of Mae Soon reservoir using fi ve different media (nutrient, Luria-Bertani, mineral salt, tryptic soy, and peptone yeast extract). Fifty-four facultative anaerobic bacterial isolates were obtained. Seven isolates showed their potential as biosurfactant producers in the drop-collapse assay. Based on 16S rRNA gene analysis, six of the biosurfactant-producing bacterial isolates belonged to the species Bacillus licheniformis and one belonged to the species B. subtilis. The biosurfactant producers and microbial community in the oil sands were determined using Denaturing Gradient Gel Electrophoresis (DGGE). Interestingly, DGGE bands corresponding to bacteria belonging to the genus Geobacillus sp. were detected. Overall, the results obtained from this work showed that indigenous bacteria in Mae Soon reservoir oil well were prospective for use in MEOR.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2023.024","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Microbial Enhanced Oil Recovery (MEOR) is an effective alternative method for oil recovery in reservoirs using microorganisms or their secondary metabolites. This research aimed to evaluate the indigenous bacteria from Mae Soon reservoir by culture-dependent and culture-independent methods and to investigate the potential of biosurfactant-producing bacteria using a drop-collapsed assay. Indigenous bacteria were isolated from the oil sands of the reserved core of Mae Soon reservoir using fi ve different media (nutrient, Luria-Bertani, mineral salt, tryptic soy, and peptone yeast extract). Fifty-four facultative anaerobic bacterial isolates were obtained. Seven isolates showed their potential as biosurfactant producers in the drop-collapse assay. Based on 16S rRNA gene analysis, six of the biosurfactant-producing bacterial isolates belonged to the species Bacillus licheniformis and one belonged to the species B. subtilis. The biosurfactant producers and microbial community in the oil sands were determined using Denaturing Gradient Gel Electrophoresis (DGGE). Interestingly, DGGE bands corresponding to bacteria belonging to the genus Geobacillus sp. were detected. Overall, the results obtained from this work showed that indigenous bacteria in Mae Soon reservoir oil well were prospective for use in MEOR.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.