Protein Adsorption and Biocompatibility of Porous Silk Fiboin Films

Proceedings of the ... International Conference on Biomedical Engineering and Informatics. International Conference on Biomedical Engineering and Informatics Pub Date : 2009-10-30 DOI:10.1109/BMEI.2009.5305691

ZiLing Sun, L. Bai, Guoping Guan, KuiHua Zhan, HongQin Dai

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Abstract

Silk fibroins are widely used in skin wound healing and tissue engineering scaffolds. This study sought to observe proteins adsorption and biocompatibility. Bradford protein assay revealed that the amount of adsorbed proteins was significantly less than that on PVA. The Sodium Dodecyl Sulfate Polyacrylamidege Electrophoresis (SDS-PAGE) indicated that more proteins adsorbed onto PVA than that PSFFs. Identification of adsorbed proteins by Western blotting revealed that there was lesser amount of fibrinogen on PSFFs than on PVA. Histologic observation after 10 days PSFFs implantation in shin and muscle of rat showed that some capillaries and microvessels grew in PSFFs, PSFFs and peri- tissues were mixed with each other, many fibroblasts, myocytes and lipocytes could be observed in PSFFs due to good biocompatibility. Keywords—protein adsorption; fibrinogen; Porpous Silk Fibroin Films (PSFFs)

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多孔丝质纤维蛋白膜的蛋白质吸附和生物相容性

丝素蛋白广泛应用于皮肤创面愈合和组织工程支架。本研究旨在观察蛋白质的吸附和生物相容性。Bradford蛋白分析表明，吸附蛋白的数量明显少于PVA。十二烷基硫酸钠聚丙烯酰胺电泳(SDS-PAGE)表明PVA吸附的蛋白质比psff多。Western blotting对吸附蛋白进行鉴定，发现psff上的纤维蛋白原含量低于PVA。在大鼠胫骨和肌肉中植入PSFFs 10 d后的组织学观察表明，PSFFs中出现了一些毛细血管和微血管，PSFFs与周围组织相互混合，由于具有良好的生物相容性，PSFFs中可观察到许多成纤维细胞、肌细胞和脂肪细胞。Keywords-protein吸附;纤维蛋白原;porporous Silk Fibroin薄膜(PSFFs)

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Proceedings of the ... International Conference on Biomedical Engineering and Informatics. International Conference on Biomedical Engineering and Informatics

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