Transcriptome sequencing reveals non-coding RNAs respond to porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in Kele piglets.

IF 2.7 3区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Animal Science and Technology Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI:10.5187/jast.2023.e46
Jing Zhang, Chunping Zhao, Min Yao, Jing Qi, Ya Tan, Kaizhi Shi, Jing Wang, Sixuan Zhou, Zhixin Li
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引用次数: 0

Abstract

Co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and Haemophilus parasuis (HPS) has severely restricted the healthy development of pig breeding. Exploring disease resistance of non-coding RNAs in pigs co-infected with PRRSV and HPS is therefore critical to complement and elucidate the molecular mechanisms of disease resistance in Kele piglets and to innovate the use of local pig germplasm resources in China. RNA-seq of lungs from Kele piglets with single-infection of PRRSV or HPS and co-infection of both pathogens was performed. Two hundred and twenty-five differentially expressed long non-coding RNAs (DElncRNAs) and 30 DEmicroRNAs (DEmiRNAs) were identified and characterized in the PRRSV and HPS co-infection (PRRSV-HPS) group. Compared with the single-infection groups, 146 unique DElncRNAs, 17 unique DEmiRNAs, and 206 target differentially expressed genes (DEGs) were identified in the PRRSV-HPS group. The expression patterns of 20 DEmiRNAs and DElncRNAs confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) were consistent with those determined by high-throughput sequencing. In the PRRSV-HPS group, the target DEGs were enriched in eight immune Gene Ontology terms relating to two unique DEmiRNAs and 16 DElncRNAs, and the unique target DEGs participated the host immune response to pathogens infection by affecting 15 immune-related Kyoto Encyclopedia of Genes and Genomes enrichment pathways. Notably, competitive endogenous RNA (ceRNA) networks of different groups were constructed, and the ssc-miR-671-5p miRNA was validated as a potential regulatory factor to regulate DTX4 and AEBP1 genes to achieve innate antiviral effects and inhibit pulmonary fibrosis by dual-luciferase reporter assays. These results provided insight into further study on the molecular mechanisms of resistance to PRRSV and HPS co-infection in Kele piglets.

转录组测序揭示了非编码 RNA 对科尔仔猪猪繁殖与呼吸综合征病毒和寄生嗜血杆菌共同感染的反应。
猪繁殖与呼吸综合征病毒(PRRSV)和寄生嗜血杆菌(HPS)的双重感染严重制约了养猪业的健康发展。因此,探索猪繁殖与呼吸综合征病毒(PRRSV)和寄生嗜血杆菌(HPS)共感染猪非编码 RNA 的抗病性,对于补充和阐明可乐猪抗病分子机制,创新中国地方猪种质资源的利用至关重要。研究人员对单株感染PRRSV或HPS以及同时感染两种病原的可乐仔猪肺部进行了RNA-seq分析。在PRRSV和HPS共感染组(PRRSV-HPS)中鉴定并鉴定了225个差异表达的长非编码RNA(DElncRNA)和30个差异表达的微RNA(DEmiRNA)。与单感染组相比,PRRSV-HPS 组鉴定出了 146 个独特的 DElncRNAs、17 个独特的 DEmiRNAs 和 206 个目标差异表达基因(DEGs)。通过实时定量聚合酶链反应(RT-qPCR)确认的 20 个 DEmiRNA 和 DElncRNA 的表达模式与高通量测序确定的表达模式一致。在PRRSV-HPS组中,目标DEGs富集在与2个独特DEmiRNAs和16个DElncRNAs相关的8个免疫基因本体术语中,独特的目标DEGs通过影响15个与免疫相关的《京都基因与基因组百科全书》富集通路,参与了宿主对病原体感染的免疫应答。值得注意的是,研究人员构建了不同群体的竞争性内源性RNA(ceRNA)网络,并通过双荧光素酶报告实验验证了ssc-miR-671-5p miRNA是调控DTX4和AEBP1基因以实现先天性抗病毒效应和抑制肺纤维化的潜在调控因子。这些结果为进一步研究可乐仔猪对 PRRSV 和 HPS 共感染的抵抗力的分子机制提供了启示。
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来源期刊
Journal of Animal Science and Technology
Journal of Animal Science and Technology Agricultural and Biological Sciences-Food Science
CiteScore
4.50
自引率
8.70%
发文量
96
审稿时长
7 weeks
期刊介绍: Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare. Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication. The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).
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