Original paper Does concentration influence viability of the bronchial epithelial cell line chronically exposed to antiasthmatic drugs

Dominik Oleś, A. Szczepankiewicz, M. Wołuń-Cholewa, W. Butowska, P. Sobkowiak, Z. Kycler, Warchoł Jb, A. Bręborowicz
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引用次数: 2

Abstract

Introduction: Bronchial epithelial cell lines enable analysis of influence of different factors, such as exposure to inhaled antiasthmatic agents, on their morphology and function in vitro. Aim: To investigate whether chronic exposure to different concentrations of therapeutic agents used in the inhala - tion therapy of asthma influence bronchial epithelial cells growth and morphology. Material and methods: Bronchial epithelial cell line 16HBE14o- was used. We analyzed changes in viability between cells treated with different drug concentrations: budesonide, fluticasone propionate, salbutamol and ipratropium bromide. Observations were performed every 24 h for 4 days. Cells viability was analyzed by fluorescent staining and XTT assay. Results: An inverse correlation between drug concentration and cells viability was observed. The only exception was ipratropium bromide which was toxic at all studied concentrations. Steroids at the two highest concentrations led to a significant decrease in cells viability with fluticasone propionate being more potent than budesonide. Incuba - tion with salbutamol also demonstrated decreased cells viability at the two highest concentrations. Ipratropium bromide was toxic for the bronchial cells at all concentrations leading to a significant decrease in cells viability. Conclusions: Chronic exposure to the highest concentrations of steroids or β2-agonists decreases viability of epithe - lial cells, whereas ipratropium bromide has the strongest influence on cell viability regardless of its concentration.
浓度是否影响支气管上皮细胞系长期暴露于平喘药的活力
简介:支气管上皮细胞系可以在体外分析不同因素(如吸入平喘药)对其形态和功能的影响。目的:探讨慢性暴露于不同浓度的哮喘吸入治疗药物对支气管上皮细胞生长和形态的影响。材料与方法:支气管上皮细胞系16HBE14o-。我们分析了不同药物浓度(布地奈德、丙酸氟替卡松、沙丁胺醇和溴化异丙托品)处理后细胞活力的变化。每24 h观察一次,连续观察4天。采用荧光染色和XTT法检测细胞活力。结果:药物浓度与细胞活力呈负相关。唯一的例外是异丙托溴铵,在所有研究浓度下都是有毒的。两种最高浓度的类固醇导致细胞活力显著下降,丙酸氟替卡松比布地奈德更有效。用沙丁胺醇孵育也显示在两个最高浓度下细胞活力下降。异丙托品溴化剂在不同浓度下对支气管细胞均有毒性,导致细胞活力显著降低。结论:长期暴露于最高浓度的类固醇或β2激动剂会降低上皮细胞的活力,而溴化异丙托品对细胞活力的影响最大,无论其浓度如何。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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