FACTORS EFFECTING PROCAINE HYDROCHLORIDE ELECTROMIGRATION UNDER CONDITIONS OF CAPILLARY ELECTROPHORESIS

Abstract

The article discusses the influence of various factors on procaine hydrochloride electromigration under conditions of electrophoretic separation by capillary electrophoresis. The rate of voltage rise in the capillary electrophoresis system has been studied and a device making it possible to measure the potential in the system independent of the program software has been developed. The influence of the difference in hydrostatic pressure at the inlet and outlet of the quartz capillary and the difference in the approach to the analysis when changing the base electrolyte are investigated. The influence of the sample injected zone length on reproducibility of assay results of procaine hydrochloride has been studied. In order to improve separation efficiency and reproducibility of quantitative analysis results it is recommended to replace the base electrolyte after each analysis, as well as to inject the sample avoiding short injection values and high pressure. The values of apparent mobility of the test substance which can be used in the development of methods for procaine hydrochloride assay by capillary electrophoresis are calculated. The research results obtained can be applied in chemical and pharmaceutical laboratories as well as in scientific research.