In Vitro Study of Experimental Ag-In Alloys with Embryo Stem Cell Test Protocol and Whole Embryo Culture

Abstract

127 INTRODUCTION Using the Embryo Stem Cell Test (EST) protocol , a embryotoxicity test with mouse ES-D3 cells, embryotoxicity was noted for several indium compounds . The EST protocol has already been validated in Europe, demonstrating a strong correlation with human teratogenicity . Although various indiumcontaining alloys have already been used for dental alloys , no report has been published on their embryotoxicity risk. Using Ag-20% In and Ag-Pd-Au-Cu In Vitro Study of Experimental Ag-In Alloys with Embryo Stem Cell Test Protocol and Whole Embryo Culture