Genetic diversity in - chilli (Capsicum annuum L.) based on microsatellite markers: An evaluation of Bangladeshi germplasm

Acta agriculturae Slovenica Pub Date : 2022-12-30 DOI:10.14720/aas.2022.118.4.2511

Md. Rezwan Molla, Iftekhar U. Ahmed, M. Rohman, Mohammad Amdadul Haque, S. Hossain, L. Hassan

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引用次数: 2

Abstract

Genetic diversity analysis is a pre-requisite to develop improve variety of any crop. Hence, 39 SSR markers were used to analyze the genetic diversity of local chilli cultivars. PCR-amplified microsatellite loci were shown to be polymorphic in all investigated cultivars. The locus, CAMS-647 produced the highest number of alleles (8) ranging in size from 188 to 279 bp. PIC values for 39 primers ranged from 0.099 for the locus Hpms 1-165 to 0.806 for the locus CAMS-679. All of the SSRs examined were informative in characterizing the genotypic variance of the samples while 12 were more informative with higher PIC values (> 0.6). There was a wide range of genetic diversity varied from 0.117 (HpmsE075) to 0.806 (CAMS-647), whereas the highest (1.713) and the lowest (0.205) value of Shannon’s Information Index was registered in the locus CAMS-679 and Hpms 1-165, respectively. There was a higher degree of genetic differentiation (0.927) and a lower amount of gene flow (0.010). Nei’s genetic distance (GD) varied from 0.100 to 0.990. Among 96 cultivars, 55 had distinct status in the dendrogram with higher GD values (> 0.6), while 41 cultivars showed a close relationship and yielded lower GD values.

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基于微卫星标记的辣椒遗传多样性:孟加拉国种质资源的评价

遗传多样性分析是培育作物良种的先决条件。利用39个SSR标记对辣椒品种的遗传多样性进行了分析。pcr扩增的微卫星位点在所有被调查的品种中都显示出多态性。基因座CAMS-647产生的等位基因数量最多(8个)，大小在188 ~ 279 bp之间。39条引物的PIC值从位点Hpms 1-165的0.099到位点CAMS-679的0.806不等。所有检测到的ssr在描述样本的基因型差异方面都具有信息性，而12个ssr的PIC值越高(> 0.6)，信息就越丰富。遗传多样性范围为0.117 (HpmsE075) ~ 0.806 (CAMS-647)，而Shannon信息指数最高(1.713)和最低(0.205)分别出现在CAMS-679和Hpms 1-165位点。遗传分化程度较高(0.927)，基因流量较低(0.010)。Nei的遗传距离(GD)在0.100 ~ 0.990之间变化。在96个品种中，55个品种在树形图中地位明显，GD值较高(>.6)，41个品种关系密切，GD值较低。

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来源期刊

Acta agriculturae Slovenica Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： Acta agriculturae Slovenica is a scientific journal published quarterly by the Agronomy, and the Zootechnical Departments of the Biotechnical Faculty, University of Ljubljana, Slovenia. Source of high quality research from the field of agronomy, zootechnics, applied biology and related fields from Slovenia and other countries.