Design, Synthesis, Characterisation and Pharmacological Evaluation of Some Novel Substituted Pyrimidine 2-one Derivatives

Abstract

2,3-dihydropyrimidin-2(1H)-one derivatives were known to undergo cyclo-condensation of benzaldehyde, urea and ethyl acetoacetate and evaluate their antioxidant and in vitro anti-inflammatory activity. Dihydropyrimidinones are mainly synthesized by Biginelli reaction, which involves cyclo condensation of benzaldehyde, urea and ethyl acetoacetate. The prepared compounds were characterized by noting their melting point, thin layer chromatography ultraviolet spectroscopy, infrared spectroscopy, nuclear magnetic resonance and Mass spectroscopy and were scrutinized for its in vitro anti-inflammatory activity and antioxidant activity by in vitro cell culture studies. The melting point, thin layer chromatography and ultraviolet spectroscopy of the synthesized compounds were found to be pure and identified chemically. The molecular structure and molecular mass of compounds were confirmed by Infrared, nuclear magnetic resonance and Mass spectroscopy. The IC50 value of compound 1 and compound 2 by hydrogen peroxide method was found to be 30.25 μg/ml and 26.06 μg/ml respectively. The IC50 value of compound 1 and compound 2 by DPPH assay method was found to be 27.872 μg/ml and 25.467 μg/ml respectively. Synthesized compounds also show anti-inflammatory activity. The result obtained in this research work is clearly indicating that the synthesized molecules possess both antioxidant activity and anti-inflammatory activity.