Obtaining Complete Human Proteomes.

IF 7.7 2区生物学 Q1 GENETICS & HEREDITY

Annual review of genomics and human genetics Pub Date : 2022-04-19 DOI:10.1146/annurev-genom-112921-024948

Ana Martínez-Val, Ulises H. Guzmán, J. Olsen

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引用次数: 6

Abstract

Proteins are the molecular effectors of the information encoded in the genome. Proteomics aims at understanding the molecular functions of proteins in their biological context. In contrast to transcriptomics and genomics, the study of proteomes provides deeper insight into the dynamic regulatory layers encoded at the protein level, such as posttranslational modifications, subcellular localization, cell signaling, and protein-protein interactions. Currently, mass spectrometry (MS)-based proteomics is the technology of choice for studying proteomes at a system-wide scale, contributing to clinical biomarker discovery and fundamental molecular biology. MS technologies are continuously being developed to fulfill the requirements of speed, resolution, and quantitative accuracy, enabling the acquisition of comprehensive proteomes. In this review, we present how MS technology and acquisition methods have evolved to meet the requirements of cutting-edge proteomics research, which is describing the human proteome and its dynamic posttranslational modifications with unprecedented depth. Finally, we provide a perspective on studying proteomes at single-cell resolution. Expected final online publication date for the Annual Review of Genomics and Human Genetics, Volume 23 is October 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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获得完整的人类蛋白质组。

蛋白质是基因组编码信息的分子效应器。蛋白质组学旨在了解蛋白质在生物学背景下的分子功能。与转录组学和基因组学相比，蛋白质组学的研究提供了对蛋白质水平编码的动态调控层的更深入的了解，如翻译后修饰、亚细胞定位、细胞信号传导和蛋白质-蛋白质相互作用。目前，基于质谱(MS)的蛋白质组学是在系统范围内研究蛋白质组学的首选技术，有助于临床生物标志物的发现和基础分子生物学。质谱技术正在不断发展，以满足速度、分辨率和定量准确性的要求，从而能够获得全面的蛋白质组。在这篇综述中，我们介绍了质谱技术和获取方法是如何发展到满足尖端蛋白质组学研究的要求的，这些研究正在以前所未有的深度描述人类蛋白质组及其动态翻译后修饰。最后，对单细胞分辨率下蛋白质组学的研究进行了展望。预计《基因组学与人类遗传学年度评论》第23卷的最终在线出版日期为2022年10月。修订后的估计数请参阅http://www.annualreviews.org/page/journal/pubdates。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annual review of genomics and human genetics 生物-遗传学

CiteScore

14.90

自引率

1.10%

发文量

期刊介绍： Since its inception in 2000, the Annual Review of Genomics and Human Genetics has been dedicated to showcasing significant developments in genomics as they pertain to human genetics and the human genome. The journal emphasizes genomic technology, genome structure and function, genetic modification, human variation and population genetics, human evolution, and various aspects of human genetic diseases, including individualized medicine.