Identification of Synthetic Cannabinoid 5F-ADB (5F-MDMB-PINACA) and Its Metabolite in Urine Sample Using Liquid Chromatography – High Resolution Mass Spectrometer (LC-HRMS)

The Journal of Pure and Applied Chemistry Research Pub Date : 2020-08-31 DOI:10.21776/UB.JPACR.2020.009.02.537

Rieska Dwi Widayati, T. Tanti, Erlana Nindya Maulida, Martin Luther Silubun

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Abstract

Synthetic cannabinoids are commonly known as Gorillas Tobacco, Hanomans Tobacco or Ganeshas Tobacco in Indonesia. Those products are camouflaged as a tobacco related to the number of smokers in Indonesia. The 5F-ADB (5F-MDMB-PINACA) has become an issue since 2016. It was undetectable by conventional drug testing methodology such as immunoassay method. GC-MS as a routine method analysis is not recommended also for detecting the metabolites from biological specimen with low concentration. The paper report LC-HRMS based method for identification of 5F-ADB and its metabolites in urine sample. Various of volume injections (1, 3, and 6 µL) was studied. Sample was acidified with concentrate of HCl, then undergo extraction with EXtrelut® Column NT3 prior to LC-HRMS analysis. The full method was operated for MS/dd-MS2 identification. The 5F-ADB and its ester hydrolysis metabolite, 5F-ADB metabolite 7 (C 19 H 27 FN 3 O 3 + ) was detected in urine sample.

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液相色谱-高分辨率质谱联用(LC-HRMS)鉴定尿液中合成大麻素5F-ADB (5F-MDMB-PINACA)及其代谢物

在印度尼西亚，合成大麻素通常被称为大猩猩烟草、哈诺曼烟草或甘尼萨烟草。这些产品被伪装成与印度尼西亚吸烟者数量有关的烟草。5F-ADB (5F-MDMB-PINACA)自2016年以来一直是一个问题。免疫分析法等常规药物检测方法无法检测到。GC-MS作为常规分析方法也不推荐用于检测低浓度生物标本的代谢物。本文报道了基于LC-HRMS的尿样中5F-ADB及其代谢物的鉴定方法。研究了不同的体积注射(1、3、6µL)。样品用盐酸浓缩液酸化，然后用EXtrelut®NT3柱提取，然后进行LC-HRMS分析。采用完整的方法进行MS/dd-MS2鉴定。尿样中检测5F-ADB及其酯水解代谢物5F-ADB代谢物7 (c19h27f3o3 +)。

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The Journal of Pure and Applied Chemistry Research

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