Expression of intercellular adhesion molecule-1 in hepatic stellate cells

Lu Lungen, Zeng Min-de, Fan Jiangao, Hua Jing, L. Jiqiang, Qiu Dekai, Fan Zhuping
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Abstract

OBJECTIVE: To explore the expression of intercellular adhesion molecule-1 (ICAM-1) in hepatic stellate cells (HSC). METHODS: Via in situ perfusion with proteinase and collagenase and density-gradient centrifugation with Nycodenz, HSC were isolated and cultured from the livers of both normal Wistar rats and the livers of rats treated with carbon tetrachloride. Expression of ICAM-1 in HSC was detected by an immunohistochemistry assay and reverse transcription–polymerase chain reaction (RT-PCR). RESULTS: No ICAM-1 was expressed in the freshly isolated HSC of normal rats, but ICAM-1 was found in primary cultures of HSC at day 10 and in secondary cultures of HSC at day 7. Additionally, the intensity of the expression increased over time. Expression of ICAM-1 was observed in freshly isolated HSC from the livers of rats treated with carbon tetrachloride. CONCLUSIONS: Expression of ICAM-1 is related to the activation of HSC, hepatic inflammation and hepatic fibrogenesis.
肝星状细胞细胞间粘附分子-1的表达
目的:探讨细胞间粘附分子-1 (ICAM-1)在肝星状细胞(HSC)中的表达。方法:采用蛋白酶和胶原酶原位灌注和Nycodenz密度梯度离心,分别从正常Wistar大鼠和四氯化碳处理大鼠肝脏中分离培养HSC。采用免疫组织化学法和逆转录聚合酶链反应(RT-PCR)检测ICAM-1在HSC中的表达。结果:正常大鼠新鲜分离的HSC中不表达ICAM-1,但在第10天的HSC原代培养和第7天的HSC二次培养中发现了ICAM-1。此外,这种表达的强度随着时间的推移而增加。在四氯化碳处理的大鼠肝脏新鲜分离的HSC中观察到ICAM-1的表达。结论:ICAM-1的表达与HSC活化、肝脏炎症和肝纤维化有关。
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