Polysaccharides Chiral Stationary Phases in Liquid Chromatography

Abstract

Chiral resolution has achieved an independent identity in the separation sciences and polysaccharide CSPs are viewed as effective and efficient CSPs due to their many unique advantages. The present review article highlights the separations of chiral pharmaceuticals and drugs by liquid chromatographic modalities (high performance liquid chromatography, capillary electro‐chromatography, sub‐ and super critical fluid chromatography and thin layer chromatography) utilizing polysaccharide CSPs. Enantiomeric resolution at analytical and preparative scales and a comparison of coated and immobilized CSPs is discussed. The optimization strategies for enantiomeric separation have also been presented. The possible mechanisms of chiral resolution of racemates were also included. The commercial CSPs of all the Companies i.e., Daicel, Kromasil, Macherey Nagel, Knauer and Sepaserve have been compared and discussed. Finally, the role of these CSPs in chiral drugs development programs has also been identified.