Comparative antioxidant activity of Brucea javanica (L) Merr seed extract derived from maceration and soxhletation method

PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019 Pub Date : 2019-12-23 DOI:10.1063/1.5141312

Widia Nila Risnadewi, H. Muliasari, Candra D Hamdin, Y. Andayani

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引用次数: 2

Abstract

Free radicals are an atom or molecule that has unpaired electrons which make them reactive and unstable that caused various damages to the cells. The natural antioxidants are very needed to inhibit free radicals. Brucea javanica (L) Merr, known as “Wali”, is one of the local plants that potential as a natural antioxidants due to the content of phenolic compound. The aim of this study was to determine the antioxidant activity by using DPPH method and the total phenolic content in the ethanol extract of Wali seeds derived from maceration and soxhletation. The results of antioxidant activity measurements showed that ethanol extract of Wali seeds derived from maceration had a strong activity (IC50 of 64,703 ppm), while the extract from soxhletation had a moderate activity (IC50 of 121,739 ppm). The total phenolic compounds responsible to the antioxidant activity of ethanol extract of Wali seeds derived from maceration and soxhletation were 4.946,35 mg EAG/100g and 3.830,72 mg EAG/100g, respectively. The ethanol extract of Wali seeds derived from maceration has higher antioxidant activity and total phenolic content than the extract from soxhletation with a significant different (p<0.05).Free radicals are an atom or molecule that has unpaired electrons which make them reactive and unstable that caused various damages to the cells. The natural antioxidants are very needed to inhibit free radicals. Brucea javanica (L) Merr, known as “Wali”, is one of the local plants that potential as a natural antioxidants due to the content of phenolic compound. The aim of this study was to determine the antioxidant activity by using DPPH method and the total phenolic content in the ethanol extract of Wali seeds derived from maceration and soxhletation. The results of antioxidant activity measurements showed that ethanol extract of Wali seeds derived from maceration had a strong activity (IC50 of 64,703 ppm), while the extract from soxhletation had a moderate activity (IC50 of 121,739 ppm). The total phenolic compounds responsible to the antioxidant activity of ethanol extract of Wali seeds derived from maceration and soxhletation were 4.946,35 mg EAG/100g and 3.830,72 mg EAG/100g, respectively. The ethan...

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鸦胆子(L)种子浸渍法和索浸法提取物的抗氧化活性比较

自由基是一种带有未配对电子的原子或分子，这些电子使它们具有反应性和不稳定性，对细胞造成各种损害。我们非常需要天然抗氧化剂来抑制自由基。鸦嘴鸦(L) Merr，俗称“Wali”，由于其酚类化合物的含量，是一种具有天然抗氧化剂潜力的当地植物。本研究采用DPPH法测定瓦里籽浸渍和浸出乙醇提取物的抗氧化活性和总酚含量。抗氧化活性测定结果表明，浸渍法提取的瓦利种子乙醇提取物具有较强的抗氧化活性(IC50为64,703 ppm)，而浸渍法提取的瓦利种子提取物具有中等的抗氧化活性(IC50为121,739 ppm)。水渍法和水渍法皂角籽乙醇提取物抗氧化活性的总酚类化合物含量分别为4.946、35 mg EAG/100g和3.830、72 mg EAG/100g。浸渍法提取的瓦利籽醇提物抗氧化活性和总酚含量均高于浸渍法提取的瓦利籽醇提物，差异有统计学意义(p<0.05)。自由基是一种带有未配对电子的原子或分子，这些电子使它们具有反应性和不稳定性，对细胞造成各种损害。我们非常需要天然抗氧化剂来抑制自由基。鸦嘴鸦(L) Merr，俗称“Wali”，由于其酚类化合物的含量，是一种具有天然抗氧化剂潜力的当地植物。本研究采用DPPH法测定瓦里籽浸渍和浸出乙醇提取物的抗氧化活性和总酚含量。抗氧化活性测定结果表明，浸渍法提取的瓦利种子乙醇提取物具有较强的抗氧化活性(IC50为64,703 ppm)，而浸渍法提取的瓦利种子提取物具有中等的抗氧化活性(IC50为121,739 ppm)。水渍法和水渍法皂角籽乙醇提取物抗氧化活性的总酚类化合物含量分别为4.946、35 mg EAG/100g和3.830、72 mg EAG/100g。伊森……

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PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019

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