Antioxidant and Antihypertensive Activity of Collagen and Elastin Hydrolysate at Different Molecular Weights

Proceedings of the 5th International Conference on Chemical Investigation and Utilization of Natural Resource (ICCIUNR-2021) Pub Date : 2021-01-01 DOI:10.2991/ahcps.k.211004.004

B. Munkhuu, Lkhagvamaa Erdene, Zolzaya Bayarsukh, Enkh-Ariun Altantulga, Oyuntuya Baltsukh, Gan-Erdene Tudev, Ariun Narmandakh

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Abstract

The angiotensin-converting enzyme (ACE) inhibitory activity and antioxidant property of collagen and elastin hydrolysates, and their peptide fractions (< 5 kDa, 5-10 kDa, 10-100 kDa) were compared. The bovine raw-hide and paddywhack (Ligamentum nuchae) were used for the preparation of collagen and elastin hydrolysates, respectively. Unfractionated collagen and elastin hydrolysates (4 mg/mL) could reduce ACE activity by 61% and 58%. As a result of fractionation, ACE inhibitory activity of collagen hydrolysate was increased up to 85%, while this effect was not observed on elastin hydrolysate. Elastin hydrolysate showed intense radical scavenging abilities through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-Ethylbenzthiazolin-6-Sulfonic Acid (ABTS) assay. The collagen hydrolysate obtained from the bovine rawhide has more perspective for the bioactive food additive having ACEI activity in comparison with the elastin hydrolysate.

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不同分子量胶原蛋白和弹性蛋白水解物的抗氧化和降压活性

比较了胶原蛋白和弹性蛋白水解产物及其肽段(< 5 kDa、5-10 kDa、10-100 kDa)的血管紧张素转换酶(ACE)抑制活性和抗氧化性能。分别以牛生皮和牛颈韧带为原料制备胶原蛋白和弹性蛋白水解物。未分离的胶原和弹性蛋白水解物(4mg /mL)可使ACE活性分别降低61%和58%。经分离后，胶原水解产物的ACE抑制活性提高了85%，而弹性蛋白水解产物则没有这种作用。通过2,2-二苯基-1-吡啶肼(DPPH)和2,2 ' -氮化氮-(3-乙基苯并噻唑-6-磺酸(ABTS)测定，弹性蛋白水解物显示出较强的自由基清除能力。与弹性蛋白水解物相比，从牛生皮中提取的胶原蛋白水解物更有可能作为具有ACEI活性的生物活性食品添加剂。

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Proceedings of the 5th International Conference on Chemical Investigation and Utilization of Natural Resource (ICCIUNR-2021)

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