High-fat diet affects measures of skeletal muscle contractile performance in a temperature specific manner but does not influence regional thermal sensitivity.

Abstract

The present study examined if 20-weeks high-fat diet (HFD) consumption had a temperature specific effect on the contractile performance and regional thermal sensitivity of isolated mouse soleus (SOL) and diaphragm (DIA) muscle. Four-week-old female CD-1 mice were randomly selected to consume either a standard laboratory diet or a standard laboratory diet in conjunction with a HFD for 20-weeks. Peripheral SOL and core DIA were isolated from each animal and maximal isometric force and work loop power were assessed at 20⁰C, 28⁰C, 35⁰C and 40⁰C. Increasing temperature to 35⁰C resulted in greater isometric stress, lower activation and relaxation time and higher work loop power in both muscles. A further increase in temperature to 40⁰C did not affect isometric force but increased work loop power output of the SOL. Conversely, isometric force of the DIA was reduced and work loop power maintained when temperature was increased to 40⁰C. HFD consumption resulted in greater isometric force and absolute work loop power of the SOL and reduced isometric stress of the DIA, effects that were less apparent at lower temperatures. When the relationship between temperature and each measure of contractile function was examined by linear regression, there was no difference in slope between the control or HFD groups for either SOL or DIA. These results indicate that whilst contractile function initially increases with temperature, the temperature to elicit maximal performance is muscle and contractile mode-specific. Furthermore, HFD effects on contractile function are temperature specific, but HFD does not influence the relationship between temperature and performance.