Antibacterial activity of lactobacilli from buffalo milk and yoghurt in Bandar-e Gaz, North-West Iran

Q3 Veterinary

BULGARIAN JOURNAL OF VETERINARY MEDICINE Pub Date : 2022-01-01 DOI:10.15547/bjvm.2020-0073

F. Bagheri, A. Ahani Azari, H. Koohsari

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引用次数: 1

Abstract

A cross-sectional study was conducted from April to June 2016 to determine the inhibitory effect of Lactobacillus strains isolated from buffalo milk and yogurt in Bandar-e Gaz, Golestan province, North-West Iran. The raw milk and yogurt samples were collected and cultivated on MRS medium by anaerobical incubation at 37 °C for 48 h. The suspected colonies were identified on the basis of Gram’s staining and conventional biochemical tests. The antibacterial activity of the cell-free supernatant extracted from Lactobacillus strains was determined using the agar well diffusion method against standard strains Escherichia coli ATCC 11303, Staphylococcus aureus ATCC 29213, Bacillus cereus ATCC 19115, Listeria monocytogenes ATCC 19111, E. coli and S. aureus isolates from local cheese samples. A total of 10 Lactobacillus spp. were isolated and identified as L. plantarum, L. casei, L. acidophilus. L. plantarum A1 and L. acidophilus R1 exhibited relatively strong inhibitory effect against S. aureus and B. cereus, respectively. Both isolates had no inhibitory effect against L. monocytogenes. L. casei B1 showed moderate inhibitory effects against L. monocytogenes. This study showed that Lactobacilli from buffalo dairy products had good inhibitory activity towards Gram-positive indicator organisms and were one of the best choices to control these pathogens in food products.

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伊朗西北部班达尔加兹地区水牛奶和酸奶中乳酸菌的抗菌活性

2016年4月至6月进行了一项横断面研究，以确定从伊朗西北部戈列斯坦省班达尔加兹市水牛奶和酸奶中分离的乳杆菌菌株的抑制作用。收集原料乳和酸奶样品，在MRS培养基上37℃厌氧培养48 h，通过革兰氏染色和常规生化试验鉴定可疑菌落。采用琼脂孔扩散法测定乳酸菌无细胞上清液对标准菌株大肠杆菌ATCC 11303、金黄色葡萄球菌ATCC 29213、蜡样芽孢杆菌ATCC 19115、单核增生李斯特菌ATCC 19111、大肠杆菌和金黄色葡萄球菌的抑菌活性。共分离到10株乳杆菌属，鉴定为植物乳杆菌、干酪乳杆菌、嗜酸乳杆菌。L. plantarum A1和L. acidophilus R1分别对金黄色葡萄球菌和蜡样芽孢杆菌表现出较强的抑制作用。两株菌株对单核增生乳杆菌均无抑制作用。干酪乳杆菌B1对单核增生乳杆菌有中等抑制作用。本研究表明，水牛乳制品乳酸菌对革兰氏阳性指示菌具有良好的抑制活性，是控制食品中革兰氏阳性指示菌的最佳选择之一。

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来源期刊

BULGARIAN JOURNAL OF VETERINARY MEDICINE Veterinary-Veterinary (all)

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

15 weeks

期刊介绍： BJVM is a no-fee open-access scientific quarterly journal which covers topics related to both fundamental and applied aspects of veterinary medicine and to closely connected subjects with it. The journal publishes original papers, short communications and reviews.