Skin Whitening Efficacy of Mixed Extracts of Crassostrea gigas, Trichosanthes cucumeroides, Angelica dahurica and Asarum sieboldii

Abstract

Purpose: The purpose of this study is to evaluate the cosmetic functionality of mixed extracts of Crassostrea gigas, Trichosanthes cucumeroides, Angelica dahurica and Asarum sieboldii (CTAA), which are effective in skin whitening.Methods: In this study, to evaluate the cosmetic functionality of the mixed extract, 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity evaluation, superoxide dismutase (SOD)-like activity, xanthine oxidase inhibitory activity evaluation, tyrosinase inhibitory activity evaluation, melanin production evaluation, cell toxicity evaluation was performed.Results: As a result of ABTS and DPPH radical analysis, CTAA-4 was 60.7% and 59.1% at 500 μg/mL, respectively, showing the best antioxidant activity. As a result of the SOD-like activity analysis, CTAA-4 was confirmed to be 32.3±3.7% at the concentration of 500μg/mL, and in the xanthine oxidase inhibitory activity result, CTAA-4 was 29.6±3.6% at the concentration of 500 μg/mL. In the tyrosinase activity analysis result, CTAA-4 had the lowest tyrosinase activity at 59.7% at a concentration of 500 μg/mL. Melanin production was also found to be 44.6% of CTAA-4 compared to the control at a concentration of 500 μg/mL. As a result of cytotoxicity, all test substances had no cytotoxicity at a concentration of 100 to 500μg/mL as cell viability was confirmed to be more than 95%.Conclusion: As a result of the study, it was confirmed that the mixed extract developed in this study has excellent inhibitory activity of free radicals and reactive oxygen species, and has excellent skin whitening effect by inhibiting tyrosinase activity and melanin production.