Binding site and equilibrium constant of ethylenediamineplatinum(II) or triamineplatinum(II) to thymidine

Bioinorganic chemistry Pub Date : 1978-10-01 DOI:10.1016/S0006-3061(00)80027-7

Kenji Inagaki, Yoshinori Kidani

{"title":"Binding site and equilibrium constant of ethylenediamineplatinum(II) or triamineplatinum(II) to thymidine","authors":"Kenji Inagaki, Yoshinori Kidani","doi":"10.1016/S0006-3061(00)80027-7","DOIUrl":null,"url":null,"abstract":"<div>The complex of thymidine with (NH3)3Pt(OH2) or (en)Pt(OH2)2, where en = ethylenediamine, have been prepared in aqueous solution at pH = 7. The isolated complexes, corresponding to the formula [(NH3)3Pt(ThdH−1)]NO3 and (en)Pt(ThdH−1)2 where ThdH−1 = mono anion of thymidine, have been characterized by nmr, ir, and uv spectra. The binding site is the N(3) position of ThdH−1.The equilibrium constant for (NH3)3Pt(OH2) + ThdH−1 ⇌ (NH3)3Pt(ThdH−1), in which the equilibrium of binding is pH dependent, was estimated from integrated intensity ratio of the proton of position 6 of thymidine, and log K was 10.4 ± 0.1. In the case of (en)Pt(ThdH−1)2, the log K1 for (en)Pt(OH2)2 + ThdH−1 ⇌ (en)Pt(OH2)(ThdH−1) was 10.3 ± 0.1, and the log K2 for (en)Pt(OH2)(ThdH−1) + ThdH−1 ⇌ (en)Pt(ThdH−1)2 was 7.4 ± 0.1.</div>","PeriodicalId":9177,"journal":{"name":"Bioinorganic chemistry","volume":"9 4","pages":"Pages 333-343"},"PeriodicalIF":0.0000,"publicationDate":"1978-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0006-3061(00)80027-7","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioinorganic chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006306100800277","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 4

Abstract

The complex of thymidine with (NH₃)₃Pt(OH₂) or (en)Pt(OH₂)₂, where en = ethylenediamine, have been prepared in aqueous solution at pH = 7. The isolated complexes, corresponding to the formula [(NH₃)₃Pt(ThdH₋₁)]NO₃ and (en)Pt(ThdH₋₁)₂ where ThdH₋₁ = mono anion of thymidine, have been characterized by nmr, ir, and uv spectra. The binding site is the N(3) position of ThdH₋₁.

The equilibrium constant for (NH₃)₃Pt(OH₂) + ThdH₋₁ ⇌ (NH₃)₃Pt(ThdH₋₁), in which the equilibrium of binding is pH dependent, was estimated from integrated intensity ratio of the proton of position 6 of thymidine, and log K was 10.4 ± 0.1. In the case of (en)Pt(ThdH₋₁)₂, the log K₁ for (en)Pt(OH₂)₂ + ThdH₋₁ ⇌ (en)Pt(OH₂)(ThdH₋₁) was 10.3 ± 0.1, and the log K₂ for (en)Pt(OH₂)(ThdH₋₁) + ThdH₋₁ ⇌ (en)Pt(ThdH₋₁)₂ was 7.4 ± 0.1.

查看原文本刊更多论文

乙二胺铂(II)或三胺铂(II)与胸苷的结合位点和平衡常数

在pH = 7的水溶液中制备了胸腺嘧啶与(NH3)3Pt(OH2)或(en)Pt(OH2)2的络合物，其中en =乙二胺。分子式为[(NH3)3Pt(ThdH−1)]NO3和(en)Pt(ThdH−1)2，其中ThdH−1 =胸腺嘧啶的单阴离子，用nmr、ir和uv光谱对其进行了表征。结合位点是ThdH−1的N(3)位。根据胸腺嘧啶第6位质子的积分强度比，估计了(NH3)3Pt(OH2) + ThdH−1 + (NH3)3Pt(ThdH−1)的平衡常数，其结合平衡与pH有关，对数K为10.4±0.1。以(en)Pt(ThdH−1)2为例，(en)Pt(OH2)2 + ThdH−1 + (en)Pt(OH2)(ThdH−1)+ ThdH−1 + (en)Pt(ThdH−1)2的对数K1为10.3±0.1，(en)Pt(OH2)(ThdH−1)+ ThdH−1 + (en)Pt(ThdH−1)2的对数K2为7.4±0.1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioinorganic chemistry

自引率

0.00%

发文量