SEMI QUANTITATIVE RISK PROFILE OF AFLATOXIN B1 FROM AN ASPERGILLUS FLAVUS IN A DRIED SALTED FISH IN JAVA ISLAND RESELLERS

Q3 Environmental Science

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2018-08-31 DOI:10.15578/squalen.v13i2.356

I. Hidayah

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引用次数: 1

Abstract

This study aims to determine the probability of Aflatoxin B1 exposure from Aspergillus flavus in dried salted fish. The exposure of that may cause a health problems to consumers. The collecting of salted fish was conducted in several areas in Java Island, which were Tangerang, Bandung, Cirebon, Pelabuhan Ratu, Tegal, Cilacap, Banyuwangi and Tuban. Isolation and identification of Aspergillus flavus was conducted by using pour plate method on Aspergillus Flavus Parasiticus Agar (AFPA) media. Meanwhile, Measurement of Aflatoxin B1 (AFB1) content had been done by enzyme linked immunosorbent assay (ELISA). On the other hand, the occurence probability of Aflatoxin B1 risk from Aspergillus flavus was calculated by statistical probabilistic approach in @risk version 7.0 software with Monte Carlo simulation. The results of this study showed that in the consumption of salted fish for about 3.7 g/capita/day, it is probable that there are risk of 7.74 cfu/g A. flavus exposure and 0.7291 ppb aflatoxin B1 exposure in 1 g of salted fish that were taken from sampling locations. This value is still catagorized as low risk level

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爪哇岛经销商的咸鱼干中黄曲霉黄曲霉毒素b1的半定量风险概况

本研究旨在确定咸鱼干中黄曲霉暴露黄曲霉毒素B1的可能性。接触到这些物质可能会给消费者带来健康问题。在爪哇岛的几个地区进行了咸鱼的采集工作，这些地区是:坦格朗、万隆、奇勒本、佩拉布汉拉图、特加尔、奇拉卡普、班育旺吉和图班。采用倒平板法在黄曲霉寄生琼脂(AFPA)培养基上对黄曲霉进行分离鉴定。同时，采用酶联免疫吸附法(ELISA)测定黄曲霉毒素B1 (AFB1)含量。另一方面，在@risk version 7.0软件中，通过蒙特卡罗模拟，采用统计概率法计算黄曲霉B1风险的发生概率。本研究结果表明，人均每天食用约3.7 g的咸鱼，每g取样地点的咸鱼可能有7.74 cfu/g黄曲霉毒素暴露风险和0.7291 ppb黄曲霉毒素B1暴露风险。这个值仍然被归类为低风险级别

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