EVALUATION OF ANTIMICROBIAL AND TOXICITY OF DIFFERENT FRACTIONS OF DILLENIA INDICA LINN. BARK EXTRACT

Abstract

The methanolic extract along with some organic soluble fractions of the bark of Dillenia indica Linn. were tested against four Gram-positive and seven Gram-negative bacteria and against three pathogenic fungi. N-Hexane and dichloromethane fractions showed remarkable activities against all the tested bacteria but n-Hexane fraction showed highest activity against Shigella dysenteriae and its zone of inhibition was 15.51 ± 0.75 mm. Other showed moderate or little activity. Methanol crude extract showed highest activity against fungus Candida albicans with a zone of inhibition 13.13 ± 1.75 mm. Lowest minimum inhibitory concentration (MIC) values were observed in n-Hexane fraction against Shigella dysenteriae  and Staphylococcus aureus and were 0.312 in both cases. Lowest LC 50 value 19.02 ± 1.16 of n-Hexane fraction indicated the highest toxicity incomparison with the other fractions.