Conditions for the formation of stable probiotic emulsions of wheat germ oil

N. Rodionova, E. Popov, N. Zakharova, V. S. Zakharov, D. M. Vyrodov, A. Rodionov
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Abstract

The results of the formation of emulsions of a biologically active additive - wheat germ oil in a defatted fermented milk medium fermented by a consortium of probiotic microorganisms B. bifidum, B. longum, B. adolescentis, L. casei, L. rhamnosus, L. acidophilus, L. plantarum, L. fermentum. The dispersion medium contained at least 109 CFU in 1 g, the pH was 3.65-4.75, the titratable acidity was 120-130oT. The possibility of obtaining stable emulsions with a concentration of wheat germ oil up to 50% is shown. An increase in the capacity and stability of emulsions was established in 1.1-5.9, 1.3-10.6, 1.5-4.0, 1.7-4.7, 1.3-9.7, 1.2 -5.1 when introduced into the dispersion medium 0.5-3.5% of egg white, xanthan gum, egg powder, lecithin, guar gum, skimmed milk powder, respectively. The effect of the speed of rotation of the working body of the emulsifier in the range of 1000-3000 rpm on the emulsifying ability of the dispersion medium and the sedimentation stability of emulsions has been established. An approximating dependence of the emulsifying ability on the concentration of the emulsifier was obtained, taking into account the linear, quadratic and cubic influence of the factor. The results obtained provide a scientific basis for the process of obtaining biologically highly active probiotic emulsions based on wheat germ oil for the alimentary correction of metabolic processes in the human body. High emulsifying ability and sedimentation stability are ensured by the additional introduction of emulsifying substances into the fermented dispersion medium. The resulting emulsions had high organoleptic properties, have a more pronounced biocorrective effect on a number of important body functions, due to the combination of MZP with lacto- and bifidobacteria.
小麦胚芽油稳定益生菌乳剂的形成条件
在由双歧双歧杆菌、长芽孢杆菌、青春期芽孢杆菌、干酪乳杆菌、鼠李糖乳杆菌、嗜酸乳杆菌、植物乳杆菌、发酵乳杆菌联合发酵的脱脂发酵乳培养基中形成具有生物活性的添加剂——小麦胚芽油的结果。分散培养基1 g至少含109 CFU, pH为3.65 ~ 4.75,可滴定酸度为120 ~ 130ot。在小麦胚芽油浓度达到50%的情况下获得稳定乳剂的可能性。在1.1-5.9、1.3-10.6、1.5-4.0、1.7-4.7、1.3-9.7、1.2 -5.1中分别加入0.5-3.5%的蛋清、黄原胶、蛋粉、卵磷脂、瓜尔胶、脱脂奶粉,乳状液的容量和稳定性均有所提高。确定了乳化剂工作体转速在1000 ~ 3000转/分范围内对分散介质的乳化能力和乳剂的沉淀稳定性的影响。考虑了乳化剂浓度的线性、二次和三次影响,得出了乳化能力与乳化剂浓度的近似依赖关系。研究结果为制备生物活性较高的小麦胚芽油益生菌乳剂,用于人体代谢过程的消化调节提供了科学依据。通过在发酵分散介质中添加乳化物质,保证了高乳化能力和沉降稳定性。由于MZP与乳酸菌和双歧杆菌的结合,所得乳剂具有高的感官特性,对许多重要的身体功能具有更明显的生物校正作用。
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