Whey Protein Regulates of Some Surface Molecules Expressed on Monocytes: Significance for Amelioration of Some Diabetes Disorders

Abstract

Objective: Whey protein (WP) is an extract of a camel milk protein that could be played an important role in diabetic wounds. Diabetes mellitus has decreased wound healing due to their effects in leukocyte adhesion molecules, CD11b and CD18, and antigen presenting co-stimulatory molecules (CD80 and CD86). This work is aimed to evaluate the role of WP in the regulation of the CD11b, CD18, CD80 and CD86 appearance on monocytes. Methods: In whole blood monocytes of control, diabetic and WP-treated diabetes rats were experienced for some surface molecules using ant-CD11b-FITC, ant-CD18-PE, ant-CD80-FITC and ant-CD18-PE for flow cytometry. Results: Expression of CD11b, CD80 and CD86 on monocytes was increased significantly in diabetic animals after five weeks in association to the control subjects and the WP-treated diabetic animals (P<0.01). In contrast, the CD18 expression by monocytes of WP-treated diabetic group was improved significantly in relationship to diabetic group which was minor than the control animals (P<0.01). Conclusions: The presented results showed that WP could be involved in down-regulation of the countenance of CD11b, CD80 and CD86, and the CD18 up-regulation on monocytes of diabetic animals. The significant finding of the investigation is the amelioration of some diabetes disorders. This study adds to the responsibility of monocytes in diabetes.