Seed viability and optimal germination conditions of Cedrus atlantica (Manetti ex Endl.) Carrière

Q4 Agricultural and Biological Sciences
Nesrine Hafidou, Zoheir Mehdadi, A. Latrèche, M. Dadach, I. Bouchaour
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引用次数: 0

Abstract

Abstract Our work is part of the ex situ conservation of the Atlas cedar (Cedrus atlantica (Manetti ex Endl.) Carrière), a threatened and endemic Pinaceae of the Maghreb. Our objectives are to evaluate the viability of seeds from three Algerian cedar groves ecotypes (Thienet El Had, Chelia, Chrea) by the 2,3,5 triphenyltetrazolium chloride (TZ) test and to elucidate the effect of continuous temperatures (15°C, 20°C, 25°C) on their germinative capacity. Moreover, the effects of some physicochemical pretreatments in breaking dormancy and improving germinability were estimated on the seeds of the cedar grove of Thienet El Had. In addition, in the same ecotype, the water stress, as simulated by using the PEG6000 (0 to −2.20 bar), was induced upon seed germination. Results of the TZ test showed variability in seeds’ viability among the three ecotypes with rates of 60, 85 and 90% in those of Chelia, Chrea and Thienet El Had, respectively. However, germination tests revealed the existence of partial dormancy in all tested seeds. Dramatic differences in the degree of dormancy were noted among the three provenances investigated. Untreated seeds of the three ecotypes exhibited maximum germination at 20°C: Thienet El Had (52%), Chelia (32%) and Chrea (7%). The final germination percentage was as high as 76% in seeds soaked in hot water (80°C) for 5 min followed by 56% in seeds treated with GA3 (150 ppm). The increase in water stress regularly decreases germination with a tolerance threshold corresponding to −2.2 bar.
大西洋杉木种子活力及最佳萌发条件(Manetti ex Endl.)卡里埃
我们的工作是阿特拉斯雪松(Cedrus atlantica (Manetti ex Endl.))迁地保护的一部分。马格里布的一种受威胁的地方性松科植物。我们的目标是通过2,3,5三苯基四唑氯(TZ)试验来评估阿尔及利亚雪松林三种生态型(Thienet El Had, Chelia, Chrea)种子的生存能力,并阐明连续温度(15°C, 20°C, 25°C)对其发芽能力的影响。在此基础上,评价了不同理化处理对松林种子破休眠和提高萌发性的影响。此外,在同一生态型中,PEG6000模拟的水分胁迫(0 ~−2.20 bar)对种子萌发有诱导作用。TZ试验结果表明,3个生态型的种子活力差异较大,Chelia、Chrea和Thienet El Had的种子活力差异分别为60%、85%和90%。然而,发芽试验显示所有被试种子都存在部分休眠。在调查的三个种源中,休眠程度有显著差异。3种生态型未经处理的种子在20℃萌发率最高:Thienet El Had(52%)、Chelia(32%)和Chrea(7%)。在热水(80°C)中浸泡5 min,种子的最终发芽率高达76%,GA3 (150 ppm)处理的种子的最终发芽率为56%。水分胁迫的增加有规律地降低萌发,其耐受阈值为−2.2 bar。
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来源期刊
Forestry Studies
Forestry Studies Agricultural and Biological Sciences-Forestry
CiteScore
0.70
自引率
0.00%
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0
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