Karakterisasi dan Penetapan Kadar Flavonoid Total Ekstrak dan Fraksi Bunga Pepaya Jantan (Carica papaya L.) dengan Spektrofotometri UV-Vis

Dilla Nur Pratiwi, Nastiti Utami, Diah Pratimasari
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Abstract

AbstractBackground: The potential of male papaya flowers (Carica papaya L.) as functional herbal ingredients, such as antioxidants, is influenced by the activity of secondary metabolites, one of them is flavonoid.Objective: This study to determine the content of flavonoid and characterization flavonoids in ethanol extract and fractions of male papaya flower (Carica papaya L.).Method: Male papaya flower was maceration using ethanol 70% then fractionated using multilevel fractionation. Identification flavonoid was tested by using the Taubeck, Wilstater, and TLC. Extract and fractions were determined the content of flavonoid using colorimetric method and identification of flavonoids based on the wavelength of cinnamoyl and benzoyl in the spectrum UV-Vis.Results: The results of the determination of flavonoid content showed that ethanol extract was the highest 0.6805 ± 0.0045 %QE and n-hexane fractions was the lowest 0.4178 ± 0.0058 %QE. Identification of flavonoids using UV-Vis spectroscopy method showed the absorption of substituted 3-OH flavonol and free 3-OH flavonol.Conclusion: The highest flavonoid content was found in the ethanol extract of male papaya flower. The types of flavonoids in the extract and fractions of male papaya flower indicated the type of flavonol based on the wavelength of cinnamoyl and benzoyl.Keywords: carica, flavonoid, cinnamoyl, benzoyl, UV-Vis Intisari Latar belakang: Potensi bunga pepaya jantan (Carica papaya L.) sebagai bahan fungsional herbal, seperti antioksidan dipengaruhi oleh aktivitas dari metabolit sekunder, salah satunya flavonoid.Tujuan: Penelitian ini bertujuan untuk mengetahui kadar flavonoid total serta karakterisasi flavonoid dalam ekstrak etanol dan fraksi bunga pepaya jantan (Carica papaya L.).Metode: Bunga pepaya jantan dilakukan maserasi dengan etanol 70%, dilanjutkan fraksinasi bertingkat. Identifikasi flavonoid dilakukan menggunakan metode Taubeck, Wilstater dan KLT. Kadar flavonoid ekstrak dan fraksi ditetapkan menggunakan metode kolorimetri serta identifikasi flavonoid berdasarkan pita cinnamoyl dan benzoyl pada spektrum UV-Vis.Hasil: Hasil penetapan kadar flavonoid menunjukkan bahwa ekstrak etanol memiliki kadar tertinggi sebesar 0,6805 ± 0,0045 %QE, serta kadar terendah dalam fraksi n-heksan sebesar 0,4178 ± 0,0058 %QE. Identifikasi flavonoid metode spektrofotometri UV-Vis menunjukkan penyerapan flavonol 3-OH substitusi dan flavonol 3-OH bebas.Kesimpulan: Kadar flavonoid tertinggi ditemukan dalam ekstrak etanol bunga pepaya. Jenis flavonoid dalam ekstrak dan fraksi bunga pepaya jantan mengindikasikan jenis flavonol berdasarkan panjang gelombang dari cinnamoyl dan benzoyl.Kata kunci : carica, flavonoid, cinnamoyl, benzoyl, UV-Vis
对UV-Vis光谱进行分类和分级
摘要背景:番木瓜雄花作为抗氧化剂等功能性草药成分的潜力受其次生代谢产物(黄酮类化合物)活性的影响。目的:测定木瓜雄花乙醇提取物和提取物中黄酮类化合物的含量及表征黄酮类化合物的含量。方法:用70%乙醇浸渍雄番木瓜花,用多级分馏法进行分馏。采用Taubeck、Wilstater、TLC鉴别黄酮类化合物。采用比色法测定提取液和馏分中黄酮类化合物的含量,并根据肉桂酰和苯甲酰在紫外-可见光谱中的波长进行黄酮类化合物的鉴定。结果:黄酮类化合物含量测定结果表明,乙醇萃取部位含量最高,为0.6805±0.0045 %QE;正己烷萃取部位含量最低,为0.4178±0.0058 %QE。紫外-可见光谱法鉴定黄酮类化合物,发现其吸收了取代的3-OH黄酮醇和游离的3-OH黄酮醇。结论:木瓜雄花乙醇提取物中黄酮类化合物含量最高。雄番木瓜花提取物和组分中黄酮类化合物的类型根据肉桂酰和苯甲酰的波长指示黄酮醇的类型。关键词:番木瓜,类黄酮,肉桂酰,苯甲酰,紫外光-可见紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光,紫外光土麻:木瓜黄酮类化合物、木瓜总黄酮、木瓜总黄酮、木瓜总黄酮、木瓜总黄酮、木瓜总黄酮、木瓜总黄酮、木瓜总黄酮、木瓜总黄酮。方法:用七成乙醇,用七成乙醇,用七成乙醇,用七成乙醇。黄酮类化合物的鉴定——孟古那坎方法。阿提拉·黄酮类ekstrak丹fraksi ditetapkan menggunakan metode kolorimetri舒达identifikasi黄酮类berdasarkan pita丹肉桂酰苯甲酰篇spektrum紫外可见。Hasil: Hasil penetapan kadar flavonoids menunjukkan bahwa ekstrak etakol memiliki kadar tertinggi sebesar 0,6805±0,0045% QE, serta kadar terendah dalam fraksi n-heksan sebesar 0,4178±0,0058% QE。用紫外-可见分光光度法鉴定黄酮类化合物,并与黄酮类化合物3-OH取代。卡达尔黄酮类化合物的制备、研究、应用、研究。Jenis flavonoids dalam ekstrak danfraksi bunga pepaya jantan mengindikasikan Jenis黄酮醇berdasarkan panjang gelombang dari肉桂酰丹苯甲酰。肉桂,黄酮类化合物,肉桂酰,苯甲酰,UV-Vis
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