Classification of White Blood Cells Based on Cell Diameter, Specific Membrane Capacitance and Cytoplasmic Conductivity Leveraging Microfluidic Constriction Channel

Abstract

This paper reports the classification of white blood cells based on cell diameter Dc, specific membrane capacitance Csm and cytoplasmic conductivity $\sigma_{\text{cy}}$ leveraging a double T-type constriction channel based microfluidic platform. As a demonstration, Dc, Csm and $\sigma_{\text{cy}}$ of granulocytes ($\mathrm{n}_{\text{cell}}=683$), monocytes ($\mathrm{n}_{\text{cell}}=203$) and lymphocytes ($\mathrm{n}_{\text{cell}}=856$) were quantified as $9.23\pm 0.60$ vs. $9.40\pm 0.54$ vs. $6.64\pm 0.41\ \mu \mathrm{m},\ 1.22\pm 0.20$ vs. $1.55\pm 0.40$ vs. $1.54\pm 0.27\ \mu \mathrm{F}/\text{cm}^{2},\ 0.35\pm 0.06$ vs. $0.53\pm 0.06$ vs. $0.46 \pm 0.06\ \mathrm{S}/\mathrm{m}$. Successful classification rates of white blood cells were quantified as 71.6% (Dc), 60.4% (Csm), 73.6% ($\sigma_{\text{cy}}$) and 95.2% (Dc & Csm & $\sigma_{\text{cy}}$).