Effect of corvitin on the level of B-lymphocytes and immunoglobulins A, M, G in the blood in the conditions of formation of contact dermatitis and pneumonia

Abstract

. Contact dermatitis (CD) and pneumonia remain the most common diseases among the pathology of the bronchopulmonary system and skin. The aim of the research was to determine the level of activity B-lymphocytes, of immunoglobulins A, M, G in the blood and to establish the corrective effect of corvitine on them in the conditions of CD and experimental pneumonia (EP). Studies were performed on 64 guinea pigs, males, weighing 180-210 g. Contact dermatitis was reproduced by the method of VA Volkova, EP was reproduced by the method of VN Shlyapnikov, TL Solodova. The content B-lymphocytes, of immunoglobulins A, M, G in the blood was determined by the method of EF Chernushenko, LS Kogosova. Corvitini was administered daily at a dose of 40 mg per 1 kg of body weight for 10 days (from the 8 th to the 18 th day) EP and CD. Animals were decapitated under ether anesthesia on on the 4 th , 8 th , 10 th and 18 th days before and after administration of this drug. It was found that CD and EP (4 th , 8 th , 10 th and 18 th days) is accompanied by an increase in Ig A content in the blood by 49,2 % (p <0.05), 76,8 % (p <0.05), 104,6 % (p <0.001) and 108,2 % (p <0.001). Ig M by 52,3 % (p <0.05), 54,6 % (p <0.05), 86,9 % (p <0.05) and 97,8 % (p <0.001), respectively; Ig G by 51,3 % (p <0.05), 68,4 % (p <0.05), 79,1 % (p <0.05) and 85,6 % (p <0.05) against control before treatment; B-lymphocytes 23,1 %; 28,5 %; 34,8 % і 42,5 % (p <0.05). The use of corvitine led to a decrease in Ig A, Ig M and Ig G, B-lymphocytes in the blood by 30,1 % (p <0.05), 34,3 % (p <0.05) and 31,5 %, 20,5 % (p <0, 05) in relation to a group of animals with EP and CD before treatment, which indicates its immunocorrective effect. Manifestation of contact dermatitis and experimental pneumonia is manifested by a consistent increase in the content of B-lymphocytes and Ig A, M, G in the blood, in the dynamics of their development with an advantage on the 18th day of the experiment. Our research results suggest, however, that these indicators play an important role in the pathogenesis of the formation of these experimental models of diseases. The use of the drug corvitin led to a decrease in the concentration of immunoglobulins and B-lymphocytes in the blood, which indicated its immunocorrective effect in CD and EP.