Separation and determination of lithium and manganese ions in healthy humans and multiple sclerosis patients based on Nano graphene oxide by Ultrasound assisted-dispersive -micro solid-phase extraction

Seyed Majid Nabipour Haghighi, Negar Motakef Kazemi
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Abstract

Lithium regulates the concentration of nitric oxide in the human body and a high dose of nitric oxide causes multiple sclerosis (MS). Also, the amount of manganese in the cerebrospinal fluid alters the metabolic reactions associated with MS. In this study, the mixture of the ammonium pyrrolidine dithiocarbonate (APDC), the hydrophobic ionic liquid [HMIM][PF6] and acetone coated on the surface of graphene oxide nanoparticles (GONPs) and used for separation Li and Mn in human samples by ultrasound assisted-dispersive-ionic liquid-micro-solid phase extraction technique (USA-DIL-μ-SPE) at pH 6.0. After extraction and back-extraction, the amount of lithium and manganese in the blood, serum and urine samples was determined by the flame and the graphite furnace atomic absorption spectroscopy (F-AAS, GF-AAS), respectively. By optimizing parameters, the LOD, Linear ranges (LR) and preconcentration factor (PF) for Li and Mn ions were obtained (0.03 mg L-1, 0.25 μg L-1), (0.1-0.4 mg L-1, 0.08-1.5 μg L-1) and 10, respectively (%RSD<5). The capacity adsorption of APDC/IL/GONPs and GONPs was achieved (148.5 mg g-1, 122.3 mg g-1) and (41.3 mg g-1, 33.7 mg g-1) for Li and Mn ions in a static system, respectively. This method was successfully validated by spiking samples and certified reference materials (CRM).
超声辅助分散微固相萃取纳米氧化石墨烯分离测定健康人及多发性硬化症患者体内锂、锰离子
锂调节人体内一氧化氮的浓度,高剂量的一氧化氮会导致多发性硬化症(MS)。此外,脑脊液中锰的含量也会改变与ms相关的代谢反应。本研究将吡咯烷二硫代碳酸铵(APDC)、疏水离子液体[HMIM][PF6]和丙酮的混合物涂覆在氧化石墨烯纳米颗粒(GONPs)表面,在pH 6.0的条件下,采用超声辅助-分散-离子液-微固相萃取技术(usda - dil -μ-SPE)分离人体样品中的Li和Mn。提取和反提取后,分别用火焰和石墨炉原子吸收光谱法(F-AAS, GF-AAS)测定血液、血清和尿液中锂和锰的含量。通过优化参数,得到Li和Mn离子的LOD、线性范围(LR)、预富集因子(PF)分别为0.03 mg L-1、0.25 μ L-1、0.1 ~ 0.4 mg L-1、0.08 ~ 1.5 μ L-1和10 (%RSD<5)。APDC/IL/GONPs和GONPs对Li和Mn离子的吸附容量分别为148.5 mg g-1、122.3 mg g-1和41.3 mg g-1、33.7 mg g-1。该方法通过加峰样品和认证标准物质(CRM)成功验证。
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