Overview on the diagnostic methods of Cryptosporidiosis in bovine calves

A. Nady
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Abstract

C ryptosporidiosis is one of the most important causes of neonatal enteritis and diarrhea in calves leading to significant morbidity and mortality rates globally. This study was conducted to highlight the diagnostic methods of cryptosporidiosis in pre-weaned cattle calves. For this purpose, a total of 88 diarrheic faecal samples of pre-waned calves at Giza and El-Fayoum governorates, Egypt, were microscopically examined using Modified Ziehl-Neelsen staining (MZN), Immunochromatographic test (ICT) and Enzyme linked Immunosorbent Assay (ELISA). The prevalence of infection was 71.59% (63/88), 73.86% (65/88), and 75.00% (66/88) for MZN, ICT, and ELISA respectively. The Sensitivity was 96.9%, 100%, and 100% whereas; Specificity was 100%, 100%, and 95.7% for MZN, ICT, and ELISA, respectively.
犊牛隐孢子虫病诊断方法综述
隐孢子虫病是导致新生儿肠炎和犊牛腹泻的最重要原因之一,在全球范围内导致显著的发病率和死亡率。本研究旨在探讨断奶前犊牛隐孢子虫病的诊断方法。为此,使用改良Ziehl-Neelsen染色法(MZN)、免疫层析法(ICT)和酶联免疫吸附法(ELISA)对埃及吉萨省和El-Fayoum省共88头预断奶犊牛的腹泻粪便样本进行了显微镜检查。MZN、ICT和ELISA的感染率分别为71.59%(63/88)、73.86%(65/88)和75.00%(66/88)。灵敏度分别为96.9%、100%和100%,而;MZN、ICT和ELISA的特异性分别为100%、100%和95.7%。
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