Highly purified dihydrofolate reductase of calf thymus

David M. Greenberg, Bui-Duy Tam, Eduard Jenny, Benjamin Payes
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引用次数: 22

Abstract

A procedure has been developed for the preparation of essentially pure dihydrofolate reductase (5,6,7,8-tetrahydrofolate: NAD(P)+ oxidoreductase, EC 1.5.1.3) from calf-thymus glands. The following criteria of purity were observed: The preparation exhibited only a single protein component by sedimentation ultracentrifugation, sedimentation equilibrium, sucrose gradient centrifugation, and Sephadex chromatography; with a mean mol. wt. of 33 500.

Electrophoresis on acrylamide gel showed only a single boundary. The pH-activity curve, relative substrate specificity, Michaelis constants of the substrates, and the SH character of the enzyme have been determined. Monovalent cations and Mg2+ enhanced the enzyme activity. Other divalent cations and sulfhydryl reagents were inhibitory. No stimulation of enzyme activity could be obtained by organic mercurials or urea.

小牛胸腺高纯度二氢叶酸还原酶
建立了一种从小牛胸腺中制备基本纯的二氢叶酸还原酶(5,6,7,8-四氢叶酸:NAD(P)+氧化还原酶,EC 1.5.1.3)的方法。通过沉降-超离心、沉降平衡、蔗糖梯度离心和葡聚糖层析等方法,所得产物仅含有单一蛋白组分;平均摩尔重量为33500。丙烯酰胺凝胶电泳显示只有单一边界。测定了酶的ph -活度曲线、相对底物特异性、底物Michaelis常数和SH特性。单价阳离子和Mg2+增强了酶的活性。其他二价阳离子和巯基试剂均有抑制作用。有机汞和尿素对酶活性没有刺激作用。
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