A novel L-fucose-binding lectin from Fenneropenaeus indicus induced cytotoxicity in breast cancer cells

Biji Chatterjee, K. Ghosh, N. Yadav, Santosh R. Kanade
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引用次数: 8

Abstract

Lectins are omnipresent in almost all life forms, being the proteins which specifically bind to carbohydrate moieties on the cell surface; they have been explored for their anti-tumour activities. In this study, we purified a fucose specific-lectin (IFL) from Fenneropenaeus indicus haemolymph using fucose-affinity column and characterized for its haemagglutination activity, carbohydrate specificity, dependency on cations and cytotoxicity against cancer cells. The lectin showed non-specificity against human erythrocytes. It was a Ca2+-dependent lectin which remained stable over wide pH and temperature ranges. The lectin showed effective dose dependent cytotoxicity against different human cancer cell lines and induced apoptosis in MCF-7 cells as evidenced by DNA ladder assay and PARP cleavage in a dose dependent manner. Moreover, an increased p21 level corresponding to cyclin D downregulation in response to IFL treatment was observed which might work as probable factors to inhibit cell growth and induce apoptosis of MCF-7 cells. Therefore, we report a novel lectin from the prawn haemolymph with high specificity for L-fucose and antiproliferative towards human cancer cells. However, further establishment of the modus operandi of this lectin is required to enable its biotechnological applications.
一种新型的L-焦点结合凝集素来自于indicus Fenneropenaeus诱导乳腺癌细胞毒性
凝集素在几乎所有的生命形式中都无处不在,是一种与细胞表面的碳水化合物部分特异性结合的蛋白质;人们已经探索了它们的抗肿瘤活性。在这项研究中,我们利用聚焦亲和柱从indicus Fenneropenaeus血淋巴中纯化了一种聚焦特异性凝集素(IFL),并对其血液凝集活性、碳水化合物特异性、阳离子依赖性和对癌细胞的细胞毒性进行了表征。凝集素对人红细胞无特异性。它是一种Ca2+依赖性凝集素,在较宽的pH和温度范围内保持稳定。DNA阶梯实验和PARP切割实验表明,凝集素对不同的人癌细胞具有剂量依赖性的细胞毒性,并能诱导MCF-7细胞凋亡。此外,在IFL处理下,p21水平升高与cyclin D下调相对应,这可能是抑制MCF-7细胞生长和诱导细胞凋亡的可能因素。因此,我们报道了从对虾血淋巴中提取的一种新的凝集素,这种凝集素具有高特异性的L-聚焦和对人类癌细胞的抗增殖作用。然而,需要进一步建立这种凝集素的操作方法,使其能够在生物技术上应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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