Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina

Eclética Química Journal Pub Date : 2019-10-01 DOI:10.26850/1678-4618eqj.v44.4.2019.p85-95

Paulo Roberto Barros Gomes, Marlucy Bezerra Oliveira, D. A. De Sousa, Jeremias Caetano da Silva, R. Fernandes, H. C. Louzeiro, Rayone Wesly Santos de Oliveira, Maria do Livramento de Paula, V. E. Mouchrek Filho, M. A. Fontenele

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引用次数: 10

Abstract

In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.

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柠檬皮挥发油对埃及伊蚊、光斑生物蛉和盐蒿的杀幼虫活性、杀软体虫活性和毒性

本文研究了柠檬皮提取油对埃及伊蚊、光斑蜗牛和盐蒿三期幼虫的杀幼虫活性、杀软体动物活性和毒性。为此，我们用加氢蒸馏法提取精油。然后，我们用气相色谱-质谱联用(GC-MS)对其成分进行了鉴定和定量。我们分别采用巴西卫生部和世界卫生组织采用的方法测试了杀幼虫和杀软体动物的活性。采用Probit法计算三种生物活性的致死浓度(LC50)，均为95%。色谱分析结果表明，柠檬烯(主要成分)含量为58.81%，α-木烯(次要成分)含量为0.11%。精油的杀虫活性、杀贝活性和毒性分别为15.48、13.05和743.35 mg∙L-1。因此，精油对埃及伊蚊和光斑蜗牛幼虫有活性，对盐纹伊蚊幼虫无毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Eclética Química Journal

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