DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region

Risqi Aprilianingsih, Baiq Farhatul Wahidah, Muhammad Rifqi Hariri
{"title":"DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region","authors":"Risqi Aprilianingsih, Baiq Farhatul Wahidah, Muhammad Rifqi Hariri","doi":"10.26740/jrba.v4n2.p69-74","DOIUrl":null,"url":null,"abstract":"Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"27 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Riset Biologi dan Aplikasinya","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26740/jrba.v4n2.p69-74","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species.
从内转录间隔区推断的扁虫的DNA条形码
Homalomena pexa是一种被绒毛的毛叶蕨,是2020年初新发现和描述的植物物种。该物种目前仅在南塔巴努里有报道。提供DNA序列的分子研究在这一初步调查中是必不可少的。本研究旨在对天南星科植物的条形码序列进行鉴定,并估计其在天南星科系统发育树上的位置。本研究利用ITS序列对H. pexa进行DNA条形码分析。测序结果显示,有效扩增了1040 bp的核苷酸。采用Neighbor-Joining方法和Kimura 2参数生成的系统进化树显示,peexa与H. atrox和H. humilis聚类,bootstrap值为97%。该研究提供并证明了ITS序列可用于天南星科植物的正确鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信