Cytotoxic activity of Boswellia dalzielii (Hutch) stem bark extract against head and neck squamous cell carcinoma of the tongue (AW8507 cell line)

A. P. Otitoju, I. Longdet, T. Alemika, A. Adegoke, V. Gota
{"title":"Cytotoxic activity of Boswellia dalzielii (Hutch) stem bark extract against head and neck squamous cell carcinoma of the tongue (AW8507 cell line)","authors":"A. P. Otitoju, I. Longdet, T. Alemika, A. Adegoke, V. Gota","doi":"10.4314/jpb.v16i2.14","DOIUrl":null,"url":null,"abstract":"Abstract Head and neck squamous cell carcinoma (HNSCC) represents the third most common cause of cancer deaths worldwide. In the past, several drugs with anticancer effects have been extracted from plants. The present research was designed to evaluate the cytotoxic potential of Boswellia dalzielii Hutch stem bark extract. Boswellia dalzielii is indigenous to West Africa and is used in ethnomedicine to treat gastrointestinal disorders and skin diseases among others. Numerous studies have investigated the antiproliferative effects of its congeners, but studies involving the in vitro cytotoxic effect of B. dalzielii extract are limited. Our objectives were to evaluate the cytotoxicity of the chloroform (CLBD); ethyl acetate (EABD); and petroleum ether (PEBD) fractions obtained from B. dalzielii stem bark ethanolic extract and their effect in the cell cycle of head and neck squamous cell carcinoma of the tongue (AW8507 cells). Cytotoxicity of CLBD, EABD and PEBD on AW8507 cells were revealed by MTT and clonogenic assays. Effects of the fractions on AW8507 cell cycle were investigated by flow cytometry. All the three B. dalzielii fractions were found to inhibit proliferation and colony formation; and arrested the AW8507 cell cycle in the G2/M phase. Keywords: Boswellia dalzielii; Cytotoxicity; Cell cycle arrest.","PeriodicalId":16803,"journal":{"name":"Journal of Pharmacy & Bioresources","volume":"33 3 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmacy & Bioresources","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/jpb.v16i2.14","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Abstract Head and neck squamous cell carcinoma (HNSCC) represents the third most common cause of cancer deaths worldwide. In the past, several drugs with anticancer effects have been extracted from plants. The present research was designed to evaluate the cytotoxic potential of Boswellia dalzielii Hutch stem bark extract. Boswellia dalzielii is indigenous to West Africa and is used in ethnomedicine to treat gastrointestinal disorders and skin diseases among others. Numerous studies have investigated the antiproliferative effects of its congeners, but studies involving the in vitro cytotoxic effect of B. dalzielii extract are limited. Our objectives were to evaluate the cytotoxicity of the chloroform (CLBD); ethyl acetate (EABD); and petroleum ether (PEBD) fractions obtained from B. dalzielii stem bark ethanolic extract and their effect in the cell cycle of head and neck squamous cell carcinoma of the tongue (AW8507 cells). Cytotoxicity of CLBD, EABD and PEBD on AW8507 cells were revealed by MTT and clonogenic assays. Effects of the fractions on AW8507 cell cycle were investigated by flow cytometry. All the three B. dalzielii fractions were found to inhibit proliferation and colony formation; and arrested the AW8507 cell cycle in the G2/M phase. Keywords: Boswellia dalzielii; Cytotoxicity; Cell cycle arrest.
乳香树皮提取物对舌头颈鳞状细胞癌(AW8507)细胞毒活性的研究
头颈部鳞状细胞癌(HNSCC)是全球癌症死亡的第三大常见原因。过去,从植物中提取了几种具有抗癌作用的药物。本研究旨在评价黄芪茎皮提取物的细胞毒潜能。Boswellia dalzielii原产于西非,在民族医学中用于治疗胃肠道疾病和皮肤病等。许多研究已经研究了其同系物的抗增殖作用,但涉及白芷提取物体外细胞毒作用的研究有限。我们的目的是评估氯仿(CLBD)的细胞毒性;乙酸乙酯;从黄皮乙醇提取物中提取石油醚(PEBD)组分及其对舌头颈鳞状细胞癌(AW8507细胞)细胞周期的影响。MTT和克隆实验显示CLBD、EABD和PEBD对AW8507细胞的细胞毒性。流式细胞术观察各组分对AW8507细胞周期的影响。3种黄芽孢杆菌组分均有抑制细胞增殖和菌落形成的作用;使AW8507细胞周期阻滞在G2/M期。关键词:乳香;细胞毒性;细胞周期阻滞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信