A Standardized Protocol for Genomic DNA Isolation from the Species of Plumbago Linn

Abstract

A good amount of DNA can be extracted from the species of Plumbago Linn. by following the standardized and modified protocol. The DNA isolated was quantified using a spectrophotometer at the absorbance of λ260 nm and λ280 nm. Both the species given the best result with a DNA yield of 23.8μg/μl and 0.895μg/μl in Plumbago zeylanica and Plumbago indica respectively and produced a clear band on the agarose gel. The estimation of purity of the obtained DNA was concluded by analyzing the ratio of λ260 nm/λ280 nm of the Genomic DNA which was 1.94 and 2.0 for P. zeylanica L. and P. indica L. respectively. These results are of high quality as it is in the range of 1.8-2.0 by the present optimized protocol. Key-wordsGenomic DNA standardized protocol plumbagin INTRODUCTION Plumbago Linn. is a genus under family Plumbaginaceae consisting 10-20 species of flowering plants. They are widely distributed in the tropics . In India the plant has a wide distribution ranging from Central India to North-east India, and various parts of Southern India. Plumbago zeylanica L. and Plumbago indica L. are the two medicinally important, morphologically different species of this genus selected for the present study. These two species has been reported from North-eastern regions of India. [2] Plumbago zeylanica L. is a multipurpose medicinal herb, commonly known as Ceylon leadwort or wild leadwort , however its trade name in India is Chitrak. In north-east India it is widely distributed in the wilds of Assam, Meghalaya. Roots are widely used for its medicinal properties. Roots contain napthaquinone derivatives, plumbagin, chloroplumbagin, biplumbagin, elliptinone, chitranone . Plumbagin shows strong antibacterial activity against Escherichia coli, Salmonella typhi and Staphylococcus aureus, and antifungal and inhibitory outcome in contradiction of Klebsiella pneumoniae, Serratia marcescens and moderate result in contradiction of Bacillus subtilis, and less low effect contrary to Proteus vulgaris and Pseudomonas aeruginosa, and aqueous extract shows less antibacterial action as associated to organic extract. Access this article online Quick Response Code Website: