Three-dimensional motion of a nanoparticle on the cell membrane observed by non-interferometric widefield optical profilometry

Chau-Hwang Lee, Chun-Chieh Wang, Chia-Wei Lee, Chia-Yun Huang, Jiunn-Yuan Lin, P. Wei
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Abstract

We directly observe three-dimensional motion of a gold nanoparticle on the membrane of a living cell by using non-interferometric widefield optical profilometry. We identify the occurrence of particle internalization and actin aggregation which indicates endocytosis.
用非干涉宽视场光学轮廓术观察纳米粒子在细胞膜上的三维运动
我们采用非干涉宽视场光学轮廓术直接观察了金纳米粒子在活细胞膜上的三维运动。我们确定颗粒内化和肌动蛋白聚集的发生,这表明内吞作用。
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