Gas Chromatography-Mass Spectrometry Analysis, Phytochemical Screening, Antioxidant and Antibacterial Activity of Methanol Root Extract of Rhaphiostylis beninensis (Hook.f.) Planch. [family ICACINACEAE] against Uropathogens

Q3 Pharmacology, Toxicology and Pharmaceutics

Biomedical and Pharmacology Journal Pub Date : 2023-06-30 DOI:10.13005/bpj/2687

Titilola Oyenike Animashaun, Morenike Olutunmbi Adeoye-Isijola, K. Naidoo, R. Coopoosamy, O. Olajuyigbe

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引用次数: 0

Abstract

In Nigeria, urinary tract infections (UTIs) are amid the most prevalent bacterial contagions, with a higher incidence rate in women. Depending on the severity of the infection, different antibiotics are used to treat UTIs. Herbal remedies have, on the other hand, been seen as the paranormal means of treating UTIs while antibiotic resistance has emerged as a problem for medics. This study examined the methanol extract of Rhaphiostylis beninensis (MRB) for its phytochemical and bioactive components, antioxidant, and antibacterial activities. From the study, the methanol extract's antioxidant activities grew stronger as concentration increased. Alkaloids, saponin, tannins, cardiac glycosides, flavonoids, and reducing sugar—giving the extract its pharmacological potential—were among the phytochemicals identified from the phytochemical screening. The GC-MS Chromatogram showed that there are 42 bioactive compounds in the MRB with the most prominent compounds being (Z,Z)-9,12-Octadecadienoic acid 19.96%, Lupeol 18.96%, cis-13-Octadecenoic acid 9.96%, n-Hexadecanoic acid 9.87%, 3',5'-Dimethoxyacetophenone 6.67%, 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol 3.21%, Octadecanoic acid 2.98%, Capsaicin 2.58%, α-Amyrin 2.53%, 9-Octadecenoic acid (Z)-, 2,3-dihydroxypropyl ester 2.49%, and Vitamin E 2.45%. The MRB showed significant minimum inhibitory concentration values against Pseudomonas putida PA25T, Escherichia coli EC3AT, Pseudomonas aeruginosa PA129AT and Serratia marcescens and a significant MBC value against Escherichia coli EC3AT. This study emphasizes the medicinal efficacy of R. beninensis root and indicated that the plant could make a promising candidate for the isolation of bioactive compounds that could be synthesized as medications for the treatment of bacteria in uropathogenic disorders.

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beninensis Rhaphiostylis (Hook.f.)甲醇根提取物的气相色谱-质谱分析、植物化学筛选及抗氧化和抗菌活性木板。抗尿路病原体

在尼日利亚，尿路感染(uti)是最普遍的细菌感染之一，妇女发病率较高。根据感染的严重程度，不同的抗生素被用于治疗尿路感染。另一方面，草药一直被视为治疗尿路感染的超自然手段，而抗生素耐药性已经成为医生的一个问题。本研究研究了beninensis (Rhaphiostylis beninensis, MRB)甲醇提取物的植物化学和生物活性成分、抗氧化和抗菌活性。从研究结果来看，甲醇提取物的抗氧化活性随着浓度的增加而增强。生物碱、皂苷、单宁、心苷、黄酮类化合物和还原糖是通过植物化学筛选确定的植物化学物质。GC-MS色谱分析结果表明，MRB中含有42种活性化合物，其中最主要的活性化合物为(Z,Z)-9,12-十八烯二烯酸19.96%，Lupeol 18.96%，顺式-13-十八烯酸9.96%，正十六烯酸9.87%，3'，5'-二甲氧基苯乙酮6.67%，4-((1E)-3-羟基-1-丙烯基)-2-甲氧基苯酚3.21%，十八烯酸2.98%，辣椒素2.58%，α-Amyrin 2.53%， 9-十八烯酸(Z)- 2,3-二羟丙基酯2.49%，维生素E 2.45%。MRB对恶臭假单胞菌PA25T、大肠埃希菌EC3AT、铜绿假单胞菌PA129AT和粘质沙雷氏菌具有显著的最低抑菌浓度值，对大肠埃希菌EC3AT具有显著的MBC值。本研究强调了本宁根的药用价值，并指出本宁根具有分离生物活性化合物的潜力，可作为治疗泌尿系统疾病细菌的药物。

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来源期刊

Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.20

自引率

0.00%

发文量

189

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