Different Methods of Oocytes Recovery for in Vitro Maturation in Nili Ravi Buffalo'S Oocytes

Abstract

The aim of this study was to investigate the recovery of oocytes by scorin and aspiration methods the cumulus oocytes complexes and denuded oocytes were collected from 2–3 mm follicles from local abattoir's ovaries Although, the scoring method yielded a significantly (P<0.0001) higher numbers (3.75 oocytes per ovary) of oocytes than the aspiration method (2.65 oocytes.ovary). However, the scoring method resulted in the large amount of debris in the medium. The debris in the culture medium may have an adverse effect the oocytes maturation in vitro. Therefore it was concluded that aspirating the avarian surface is a better method to recover to recover oocytes for in vitro studies.