Abstract 2573: The anticancer effects of the vitamin E isoform, γ-tocotrienol, and vitamin D3 act synergistically to inhibit MDA-MB-231 triple negative breast cancer (TNBC) cell proliferation and viabilityin vitro

M. R. Anwar, A. Hossian, Georgios Matthaiolampakis, P. Sylvester
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引用次数: 1

Abstract

Epidemiological studies suggest that dietary intake of some natural products may be effective in reducing the risk of breast cancer. Furthermore, various phytochemicals have been shown to interfere with cell signaling pathways involved in regulating breast cancer cell proliferation and viability. γ-Tocotrienol is a natural isoform of vitamin E that displays potent anticancer activity against a variety of cancer cell types at treatment doses that have little or no effect on normal cell growth or viability. Vitamin D3 is an abundant phytochemical which also displays potent antiproliferative and antiangiogenic activity against human breast cancer cells. Studies were conducted to examine the effects of γ-tocotrienol and vitamin D3 given alone or in combination on MDA-MB-231 triple negative breast cancer (TNBC) cellular proliferation, migration, colony formation, invasion, cell cycle progression, and apoptosis. Results showed that combined treatment with subeffective doses of γ-tocotrienol (8 μM) and vitamin D3 (10 nM) synergistically inhibited the growth of MDA-MB-231 cells, as determined by the MTT assay and isobologram analysis. Additional studies indicated that this combination treatment resulted in a significant inhibition in cancer cell migration and a significant reduction in cancer cell colony formation. Studies using the Matrigel invasion assay showed that combined treatment with subeffective doses of γ-tocotrienol and vitamin D3, significantly inhibited MDA-MB-231 invasiveness. Flow cytometry analysis showed that similar combined treatment of γ-tocotrienol and vitamin D3 significantly increased the percentage of cells found in G0/G1 phase, as compared to cells in the vehicle-treated control group. After 4 days of combined treatment with subeffective doses of γ-tocotrienol and vitamin D3, the percentage of cells in the G0/G1 phase of the cell cycle increased to 55% (p Citation Format: Md. R. Anwar, A.K.M.N. Hossian, Georgios Matthaiolampakis, Paul W. Sylvester. The anticancer effects of the vitamin E isoform, γ-tocotrienol, and vitamin D3 act synergistically to inhibit MDA-MB-231 triple negative breast cancer (TNBC) cell proliferation and viability in vitro [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2573.
摘要:维生素E异型体、γ-生育三烯醇和维生素D3协同抑制MDA-MB-231三阴性乳腺癌(TNBC)细胞的体外增殖和生存能力
流行病学研究表明,饮食中摄入一些天然产品可能有效降低患乳腺癌的风险。此外,各种植物化学物质已被证明干扰参与调节乳腺癌细胞增殖和活力的细胞信号通路。γ-生育三烯醇是维生素E的一种天然异构体,在对正常细胞生长或活力影响很小或没有影响的治疗剂量下,对多种癌症细胞类型显示出强大的抗癌活性。维生素D3是一种丰富的植物化学物质,对人类乳腺癌细胞也显示出强大的抗增殖和抗血管生成活性。研究了γ-生育三烯醇和维生素D3单独或联合给药对MDA-MB-231三阴性乳腺癌(TNBC)细胞增殖、迁移、集落形成、侵袭、细胞周期进展和凋亡的影响。结果显示,γ-生育三烯醇(8 μM)和维生素D3 (10 nM)联合亚有效剂量对MDA-MB-231细胞的生长有协同抑制作用。其他研究表明,这种联合治疗显著抑制了癌细胞的迁移,并显著减少了癌细胞集落的形成。采用Matrigel侵袭试验的研究表明,γ-生育三烯醇和维生素D3联合治疗可显著抑制MDA-MB-231的侵袭性。流式细胞术分析显示,与对照组相比,相似的γ-生育三烯醇和维生素D3联合处理显著增加了G0/G1期细胞的百分比。在用亚有效剂量γ-生育三醇和维生素D3联合治疗4天后,细胞周期中处于G0/G1期的细胞百分比增加到55% (p引用形式:Md. R. Anwar, A.K.M.N. Hossian, Georgios Matthaiolampakis, Paul W. Sylvester)。维生素E异型、γ-生育三烯醇和维生素D3的抗癌作用协同抑制MDA-MB-231三阴性乳腺癌(TNBC)细胞的体外增殖和活力[摘要]。见:美国癌症研究协会2021年年会论文集;2021年4月10日至15日和5月17日至21日。费城(PA): AACR;癌症杂志,2021;81(13 -增刊):2573。
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